bcl-x Antibody (2H12) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34531
![Western Blot: bcl-x Antibody (2H12)Azide and BSA Free [NBP2-34531]](https://resources.bio-techne.com/images/products/bcl-x-Antibody-2H12-Azide-and-BSA-Free-Western-Blot-NBP2-34531-img0002.jpg "Western Blot: bcl-x Antibody (2H12)Azide and BSA Free [NBP2-34531]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2H12
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for bcl-x Antibody (2H12) - Azide and BSA Free
Immunogen
A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-xL protein (Uniprot: Q07817)
Localization
Cytoplasmic and cell/nuclear membrane
Specificity
Recognizes a protein of 27kDa, identified as the Bcl-X protein. This monoclonal antibody shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This monoclonal antibody reacts with both Bcl-XS and Bcl-XL proteins.
Marker
Apoptosis Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32917).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for bcl-x Antibody (2H12) - Azide and BSA Free
Western Blot: bcl-x Antibody (2H12)Azide and BSA Free [NBP2-34531]
Western Blot: bcl-x Antibody (2H12) - Azide and BSA Free [NBP2-34531] - Rat and mouse neonatal cardiomyocytes. Western blot image submitted by a verified customer review.![Immunohistochemistry-Paraffin: bcl-x Antibody (2H12) - Azide and BSA Free [NBP2-34531]](https://resources.bio-techne.com/images/products/bcl-x-Antibody-2H12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34531-img0003.jpg "Immunohistochemistry-Paraffin: bcl-x Antibody (2H12) - Azide and BSA Free [NBP2-34531]")
Immunohistochemistry-Paraffin: bcl-x Antibody (2H12) - Azide and BSA Free [NBP2-34531]
Immunohistochemistry-Paraffin: bcl-x Antibody (2H12) - Azide and BSA Free [NBP2-34531] - FFPE human Hodgkin's lymphoma stained with bcl-x antibody (2H12). Note cytoplasmic and membrane staining.Applications for bcl-x Antibody (2H12) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-1ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Western Blot
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Bcl-x
Long Name
B Cell Lymphoma/Leukemia x
Alternate Names
BCL2L1, Bclx
Gene Symbol
BCL2L1
Additional Bcl-x Products
Product Documents for bcl-x Antibody (2H12) - Azide and BSA Free
Product Specific Notices for bcl-x Antibody (2H12) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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