beta 2-Microglobulin Antibody (B2M/961)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44471

Novus Biologicals, part of Bio-Techne
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NBP2-44471-0.02mg
NBP2-44471-0.1mg
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Conjugate
Catalog #
Alexa Fluor 350
NBP2-47704AF350
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Alexa Fluor 405
NBP2-47704AF405
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Alexa Fluor 488
NBP2-47704AF488
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Alexa Fluor 532
NBP2-47704AF532
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Alexa Fluor 594
NBP2-47704AF594
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Alexa Fluor 647
NBP2-47704AF647
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Alexa Fluor 700
NBP2-47704AF700
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Alexa Fluor 750
NBP2-47704AF750
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Allophycocyanin
NBP2-47704APC
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Allophycocyanin/Cy7
NBP2-47704APCCY7
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Biotin
NBP2-47704B
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CoraFluor 1
NBP2-47704CL1
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DyLight 350
NBP2-47704UV
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DyLight 405
NBP2-47704V
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DyLight 488
NBP2-47704G
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DyLight 550
NBP2-47704R
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DyLight 594
NBP2-47704DL594
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DyLight 650
NBP2-47704C
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DyLight 680
NBP2-47704FR
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DyLight 755
NBP2-47704IR
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FITC
NBP2-47704F
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HRP
NBP2-47704H
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Janelia Fluor 525
NBP2-47704JF525
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Janelia Fluor 549
NBP2-47704JF549
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Janelia Fluor 585
NBP2-47704JF585
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Janelia Fluor 635
NBP2-47704JF635
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Janelia Fluor 646
NBP2-47704JF646
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Janelia Fluor 669
NBP2-47704JF669
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mFluor Violet 450 SE
NBP2-47704MFV450
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mFluor Violet 500 SE
NBP2-47704MFV500
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mFluor Violet 610 SE
NBP2-47704MFV610
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PE
NBP2-47704PE
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PE/Atto594
NBP2-47704PEATT594
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PE/Cy5.5
NBP2-47704PECY55
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PE/Cy7
NBP2-47704PECY7
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PerCP
NBP2-47704PCP
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Key Product Details

Species Reactivity

Validated:

Human, Primate

Applications

Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # B2M/961

Concentration

0.2 mg/ml

View all beta 2-Microglobulin Antibodies »

Product Summary for beta 2-Microglobulin Antibody (B2M/961)

Immunogen

Full length recombinant human beta 2-Microglobulin protein (Uniprot: P61769 )

Reactivity Notes

Human and non-human primates.

Localization

Cell Surface and Extracellular (Secreted)

Specificity

Recognizes a protein of 12kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.

Marker

Renal Failure & Tumor Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47704)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for beta 2-Microglobulin Antibody (B2M/961)

Western Blot: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471]

Western Blot: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471]

Western Blot: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Western Blot Analysis of THP-1 and Raji cell lysates using beta 2-Microglobulin antibody (B2M/961).
Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471]

Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471]

Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Immunofluorescence Analysis of HeLa cells labeling with beta 2-Microglobulin Antibody (B2M/961) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471]

Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471]

Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with beta 2-Microglobulin Antibody (B2M/961).
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Applications for beta 2-Microglobulin Antibody (B2M/961)

Application
Recommended Usage

Flow Cytometry

0.5-1 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-4 ug/ml

Immunohistochemistry-Paraffin

0.5-1 ug/ml

Western Blot

0.5-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: beta 2-Microglobulin

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Alternate Names

B2M, beta 2Microglobulin

Gene Symbol

B2M

UniProt

P61769

Additional beta 2-Microglobulin Products

Product Documents for beta 2-Microglobulin Antibody (B2M/961)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for beta 2-Microglobulin Antibody (B2M/961)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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