beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47704
![Flow Cytometry: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]](https://resources.bio-techne.com/images/products/beta-2-Microglobulin-Antibody-B2M-961-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47704-img0002.jpg "Flow Cytometry: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]")
![Western Blot: beta 2-Microglobulin Antibody (B2M/961)Azide and BSA Free [NBP2-47704]](https://resources.bio-techne.com/images/products/beta-2-Microglobulin-Antibody-B2M-961-Azide-and-BSA-Free-Western-Blot-NBP2-47704-img0015.jpg "Western Blot: beta 2-Microglobulin Antibody (B2M/961)Azide and BSA Free [NBP2-47704]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Primate
Applications
CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # B2M/961
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free
Immunogen
Full length recombinant human beta 2-Microglobulin protein (Uniprot: P61769 )
Reactivity Notes
Human and non-human primates.
Localization
Cell Surface and Extracellular (Secreted)
Specificity
Recognizes a protein of 12kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.
Marker
Renal Failure & Tumor Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44471).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free
Western Blot: beta 2-Microglobulin Antibody (B2M/961)Azide and BSA Free [NBP2-47704]
Western Blot: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704] - Western Blot Analysis of THP-1 and Raji cell lysates using beta 2-Microglobulin antibody (B2M/961).![Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]](https://resources.bio-techne.com/images/products/beta-2-Microglobulin-Antibody-B2M-961-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47704-img0003.jpg "Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]")
Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]
Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704] - Immunofluorescence Analysis of HeLa cells labeling with beta 2-Microglobulin Antibody (B2M/961) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]](https://resources.bio-techne.com/images/products/beta-2-Microglobulin-Antibody-B2M-961-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47704-img0013.jpg "Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]")
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704] - Human Endometrial Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961)![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]](https://resources.bio-techne.com/images/products/beta-2-Microglobulin-Antibody-B2M-961-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47704-img0005.jpg "Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]")
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]](https://resources.bio-techne.com/images/products/beta-2-Microglobulin-Antibody-B2M-961-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47704-img0006.jpg "Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]")
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]](https://resources.bio-techne.com/images/products/beta-2-Microglobulin-Antibody-B2M-961-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47704-img0007.jpg "Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]")
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]](https://resources.bio-techne.com/images/products/beta-2-Microglobulin-Antibody-B2M-961-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47704-img0008.jpg "Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]")
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]](https://resources.bio-techne.com/images/products/beta-2-Microglobulin-Antibody-B2M-961-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47704-img0009.jpg "Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]")
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]](https://resources.bio-techne.com/images/products/beta-2-Microglobulin-Antibody-B2M-961-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47704-img0010.jpg "Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]")
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]](https://resources.bio-techne.com/images/products/beta-2-Microglobulin-Antibody-B2M-961-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47704-img0011.jpg "Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]")
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]](https://resources.bio-techne.com/images/products/beta-2-Microglobulin-Antibody-B2M-961-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47704-img0012.jpg "Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free [NBP2-47704]")
Applications for beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free
Application
Recommended Usage
Flow (Cell Surface)
1:10-1:1000
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: beta 2-Microglobulin
Alternate Names
B2M, beta 2Microglobulin
Gene Symbol
B2M
Additional beta 2-Microglobulin Products
Product Documents for beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free
Product Specific Notices for beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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