beta-Actin Antibody (8H10D10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47423

Novus Biologicals, part of Bio-Techne
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NBP1-47423
NBP1-47423SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chinese Hamster, Primate

Cited:

Human, Mouse, Rat

Applications

Validated:

ELISA, Flow (Intracellular), Flow Cytometry, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot

Cited:

Immunoblotting, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 8H10D10

Format

BSA Free

Concentration

1.0 mg/ml

View all beta-Actin Antibodies »

Product Specifications

Immunogen

This beta-Actin Antibody (8H10D10) was made to a synthetic peptide corresponding to amino-terminal residues of human Beta Actin, conjugated to KLH. [UniProt# P60709]

Localization

Cytoplasm, cytoskeleton.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta-Actin Antibody (8H10D10) - BSA Free

Western Blot: beta-Actin Antibody (8H10D10)BSA Free [NBP1-47423]

Western Blot: beta-Actin Antibody (8H10D10)BSA Free [NBP1-47423]

Western Blot: beta-Actin Antibody (8H10D10) [NBP1-47423] - Analysis using anti-Beta Actin mAb against human, mouse, hamster, rat, and primate cell lystate(s): (1) NIH/3T3, (2) Jurkat, (3) HeLa, (4) CHO, (5) PC12, (6) HEK293, (7) COS, (8) A549, and (9) MCF-7. A specific band can be observed at a molecular weight of approximately 43 kDa in all lanes.
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (8H10D10) - BSA Free [NBP1-47423]

Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (8H10D10) - BSA Free [NBP1-47423]

Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (8H10D10) [NBP1-47423] - Beta actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NBP1-47423) at 1:5400 dilution. Cells were stained using NorthernLights 557 conjugated anti-mouse secondary antibody (NL007) and counterstained with DAPI.
Simple Western: beta-Actin Antibody (8H10D10)BSA Free [NBP1-47423]

Simple Western: beta-Actin Antibody (8H10D10)BSA Free [NBP1-47423]

Simple Western: beta-Actin Antibody (8H10D10) [NBP1-47423] - Image shows a specific band for Beta Actin in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
View 10 more images

Applications for beta-Actin Antibody (8H10D10) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow (Intracellular)

1:200 - 1:400

Flow Cytometry

1:200-1:400

Immunoblotting

reported in scientific literature (PMID 27903531)

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Simple Western

12.5

Western Blot

1:1000-1:5000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 4 reviews rated 4.5 using NBP1-47423 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta-Actin

Actin is a highly conserved protein involved in cell motility, structure, and integrity. Six functional actin isoforms exist and were named based on their electrophoretic mobility and distribution including alpha-cardiac muscle actin (aCAA), alpha-skeletal muscle actin (aSKA), alpha-smooth muscle actin (aSMA), beta-cytoplasmic actin (bCYA), gamma-cytoplasmic actin (gCYA) and gamma-smooth muscle actin (gSMA) (1). The cytoplasmic isoforms, beta-actin and gamma-actin, are nearly identical, differing by only 4 amino acids at the N-terminus with a theoretical molecular weight of 42 kDa (2). Beta-actin is ubiquitously expressed in eukaryotic cells and considered a housekeeping gene which is frequently used as a loading control for assays involving protein detection, such as Western blotting (3,4).

References

1. Vandekerckhove J, Weber K. 1978. At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4):783-802. PMID: 745245

2. Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV. (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 27(2):108-16. PMID: 8162619

3. Holden VI, Lenio S, Kuick R, Ramakrishnan SK, Shah YM, Bachman MA. (2014) Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect Immun. 82(9):3826-36 PMID: 24980968

4. Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC. (2016) Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. 32(1):261-269. PMID: 27218433

Alternate Names

ACTB, betaActin

Entrez Gene IDs

60 (Human)

Gene Symbol

ACTB

UniProt

P60709

Additional beta-Actin Products

Product Documents for beta-Actin Antibody (8H10D10) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for beta-Actin Antibody (8H10D10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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