Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/36) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14093
![Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/36) - Azide and BSA Free Immunohistochemistry-Paraffin: Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/36) - Azide and BSA Free [NBP3-14093] -](https://resources.bio-techne.com/images/products/nbp3-14093_mouse-biotin-vitamin-b7-or-vitamin-h-mab-btn-36-azide-bsa-free-24120258193556.jpg "Immunohistochemistry-Paraffin: Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/36) - Azide and BSA Free [NBP3-14093] -")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Non-species specific
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, In-situ Hybridization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # BTN/36
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Biotinylated sheep immunoglobulin
Localization
Depends on the target
Specificity
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This monoclonal antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13907).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images
Immunohistochemistry-Paraffin: Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/36) - Azide and BSA Free [NBP3-14093] -
Immunohistochemistry-Paraffin: Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/36) - Azide and BSA Free [NBP3-14093] - Formalin-fixed, paraffin-embedded human kidney. Endogenous biotin stained with Biotin (Vitamin B7 or Vitamin H) antibody (BTN/36).Applications
Application
Recommended Usage
Flow Cytometry
0.5-1 ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-1 ug/ml
Immunohistochemistry-Paraffin
0.5-1 ug/ml
Western Blot
0.5-2 ug/ml
Application Notes
Confocal Microscopy
Immunohistochemistry (Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
In situ Nucleic Acid Hybridization
Immunohistochemistry (Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
In situ Nucleic Acid Hybridization
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Biotin (Vitamin B7 or Vitamin H)
Alternate Names
58-85-5, coenzyme R, d-biotin, Vitamin B7, vitamin H
Additional Biotin (Vitamin B7 or Vitamin H) Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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