Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/36) [mFluor Violet 500 SE]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14093MFV500
![Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/36) [mFluor Violet 500 SE]](https://resources.bio-techne.com/images/products/nbp3-14093mfv500_mouse-biotin-vitamin-b7-or-h-mab-btn-36-mfv500-se-5720231343571.png "Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/36) [mFluor Violet 500 SE] -")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Non-species specific
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, In-situ Hybridization, Western Blot
Label
mFluor Violet 500 SE (Excitation = 410 nm, Emission = 501 nm)
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # BTN/36
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Biotinylated sheep immunoglobulin
Localization
Depends on the target
Specificity
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This monoclonal antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images
Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/36) [mFluor Violet 500 SE] -
Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/36) [mFluor Violet 500 SE] - Vial of mFluor Violet 500 conjugated antibody. mFluor Violet 500 is optimally excited at 410 nm by the Violet laser (405 nm) and has an emission maximum of 501 nm.Applications
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
In-situ Hybridization
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Biotin (Vitamin B7 or Vitamin H)
Alternate Names
58-85-5, coenzyme R, d-biotin, Vitamin B7, vitamin H
Additional Biotin (Vitamin B7 or Vitamin H) Products
Product Specific Notices
mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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