BLNK Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB500-542
![Western Blot: BLNK Antibody [NB500-542]](https://resources.bio-techne.com/images/products/p_13107_1.jpg "Western Blot: BLNK Antibody [NB500-542]")
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Summary for BLNK Antibody
Immunogen
A fusion protein representing amino acids 171-356 of human BLNK.
Specificity
The polyclonal antibody reacts with mouse SLP65 / BLNK, a cytosolic adaptor protein identified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively spliced forms of human SLP65).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for BLNK Antibody
Western Blot: BLNK Antibody [NB500-542]
Western Blot: BLNK Antibody [NB500-542] - Fig. 1. Western Blotting analysis (reducing conditions) of whole cell lysate of Sp2/0 mouse myeloma cell line using polyclonal anti-mouse BLNK antibody.Applications for BLNK Antibody
Application
Recommended Usage
Western Blot
1:1000
Application Notes
Predicted molecular weight 54.7 kDa. Western Blot - Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: BLNK
Long Name
B-cell Linker Protein
Alternate Names
BASH, Ly57, SLP65
Entrez Gene IDs
17060 (Mouse)
Gene Symbol
BLNK
Additional BLNK Products
Product Documents for BLNK Antibody
Product Specific Notices for BLNK Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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