BLNK Antibody [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NB500-542B
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for BLNK Antibody [Biotin]
Immunogen
A fusion protein representing amino acids 171-356 of human BLNK.
Specificity
The polyclonal antibody reacts with mouse SLP65 / BLNK, a cytosolic adaptor protein identified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively spliced forms of human SLP65).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Applications for BLNK Antibody [Biotin]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Predicted molecular weight 54.7 kDa. Western Blot - Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (approx. pH 7.4)
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: BLNK
Long Name
B-cell Linker Protein
Alternate Names
BASH, Ly57, SLP65
Gene Symbol
BLNK
Additional BLNK Products
Product Documents for BLNK Antibody [Biotin]
Product Specific Notices for BLNK Antibody [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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