BMP-4 Antibody (7Q2U7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15376
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7Q2U7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 309-408 of human BMP-4 (P12644). RRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCR
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BMP-4 Antibody (7Q2U7)
Western Blot: BMP-4 Antibody (7Q2U7) [NBP3-15376]
Western Blot: BMP-4 Antibody (7Q2U7) [NBP3-15376] - Analysis of extracts of various cell lines, using BMP-4 Rabbit mAb (NBP3-15376) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Immunocytochemistry/ Immunofluorescence: BMP-4 Antibody (7Q2U7) [NBP3-15376]
Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (7Q2U7) [NBP3-15376] - Immunofluorescence analysis of HeLa cells using BMP-4 Rabbit mAb (NBP3-15376) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: BMP-4 Antibody (7Q2U7) [NBP3-15376]
Immunohistochemistry-Paraffin: BMP-4 Antibody (7Q2U7) [NBP3-15376] - Human colon using BMP-4 Rabbit mAb (NBP3-15376) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for BMP-4 Antibody (7Q2U7)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: BMP-4
Long Name
Bone Morphogenetic Protein 4
Alternate Names
BMP2B, BMP2B1, BMP4, MCOPS6, OFC11
Gene Symbol
BMP4
Additional BMP-4 Products
Product Documents for BMP-4 Antibody (7Q2U7)
Product Specific Notices for BMP-4 Antibody (7Q2U7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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