BNIP3 Antibody (2P7B8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15799
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2P7B8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 40-120 of human BNIP3 (NP_004043.3). AFPPPAAEAHPAARRGLRSPQLPSGAMSQNGAPGMQEESLQGSWVELHFSNNGNGGSVPASVSIYNGDMEKILLDAQHESG
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BNIP3 Antibody (2P7B8)
Western Blot: BNIP3 Antibody (2P7B8) [NBP3-15799]
Western Blot: BNIP3 Antibody (7Q7V3) [NBP3-15799] - Western blot analysis of extracts of various cell lines, using BNIP3 antibody (NBP3-15799) at 1:1000 dilution.Hela cells and NIH/3T3 cells were treated by Cobalt chloride (0.1 mM) at 37C for 4 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Immunohistochemistry-Paraffin: BNIP3 Antibody (2P7B8) [NBP3-15799]
Immunohistochemistry-Paraffin: BNIP3 Antibody (7Q7V3) [NBP3-15799] - Immunohistochemistry of paraffin-embedded rat heart using BNIP3 antibody (NBP3-15799) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for BNIP3 Antibody (2P7B8)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: BNIP3
Long Name
BCL2/Adenovirus E1B 19 Kda Protein-Interacting Protein 3-Like
Alternate Names
Nip3
Gene Symbol
BNIP3
Additional BNIP3 Products
Product Documents for BNIP3 Antibody (2P7B8)
Product Specific Notices for BNIP3 Antibody (2P7B8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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