BNIP3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56150
Key Product Details
Species Reactivity
Validated:
Human, Rat, Porcine, Chicken, Mouse (Negative), Xenopus
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
A synthetic peptide corresponding to amino acids 122-139 (SRPENIPPKEFLFKHPKR) of human NIP3 was used aas immunogen; GenBank no. gi|7669481|ref|NP_004043.2|.
Reactivity Notes
94% sequence identity with Canine protein. Does not work in mouse.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BNIP3 Antibody
![Western Blot: BNIP3 Antibody [NB100-56150]](https://resources.bio-techne.com/images/products/BNIP3-Antibody-Western-Blot-NB100-56150-img0001.jpg "Western Blot: BNIP3 Antibody [NB100-56150]")
Western Blot: BNIP3 Antibody [NB100-56150]
Western Blot: BNIP3 Antibody [NB100-56150] - BNIP3 expression in porcine whole skeletal muscle lysate. Image from verified customer review.![Immunohistochemistry-Paraffin: BNIP3 Antibody [NB100-56150]](https://resources.bio-techne.com/images/products/BNIP3-Antibody-Immunohistochemistry-Paraffin-NB100-56150-img0002.jpg "Immunohistochemistry-Paraffin: BNIP3 Antibody [NB100-56150]")
Immunohistochemistry-Paraffin: BNIP3 Antibody [NB100-56150]
Immunohistochemistry-Paraffin: BNIP3 Antibody - Unpurified [NB100-56150] - BNIP3 Antibody [NB100-56150] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human liver using BNIP3 antibody at 1:1000 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Mitochondrial staining was observed. Staining was performed by Histowiz.Applications for BNIP3 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:1000-1:5000
Western Blot
1:1000-1:2000
Reviewed Applications
Read 1 review rated 4 using NB100-56150 in the following applications:
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Whole antisera
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BNIP3
Long Name
BCL2/Adenovirus E1B 19 Kda Protein-Interacting Protein 3-Like
Alternate Names
Nip3
Gene Symbol
BNIP3
Additional BNIP3 Products
Product Documents for BNIP3 Antibody
Product Specific Notices for BNIP3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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