BNIP3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77683

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.17 mg/ml

View all BNIP3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region of the human BNIP3 protein (within residues 50-150). [Swiss-Prot Q12983]

Localization

Mitochondrion membrane. Note: Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BNIP3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: BNIP3 Antibody - BSA Free [NBP1-77683]

Immunocytochemistry/ Immunofluorescence: BNIP3 Antibody - BSA Free [NBP1-77683]

Immunocytochemistry/Immunofluorescence: BNIP3 Antibody [NBP1-77683] - BNIP3 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry: BNIP3 Antibody - BSA Free [NBP1-77683]

Immunohistochemistry: BNIP3 Antibody - BSA Free [NBP1-77683]

Immunohistochemistry: BNIP3 Antibody [NBP1-77683] - Analysis of BNIP3 in mouse kidney using DAB with hematoxylin counterstain.
Flow Cytometry: BNIP3 Antibody - BSA Free [NBP1-77683]

Flow Cytometry: BNIP3 Antibody - BSA Free [NBP1-77683]

Flow Cytometry: BNIP3 Antibody [NBP1-77683] - An intracellular stain was performed on PC12 cells with BNIP3 Antibody NBP1-77683F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.
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Applications for BNIP3 Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

2-5 ug/million cells/ml

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

reported in scientific literature (PMID 19574421)
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.17 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BNIP3

BCL2/adenovirus E1B 19kD protein-interacting protein 3 (BNIP3) is a mitochondrial protein critical for apoptotic events in response to adenovirus infection. It has been implicated in causing a necrotic-like cell death pattern in cells, induced by CD47. This effect has been seen in many cancer cells, and its mis-regulation causes tumor cells to be impervious to normal autophagic events. The tumor suppressor p53 represses the expression of BNIP3, often as a consequence of hypoxic micro-environments as seen in tumors. When BNIP3 is correctly expressed and activated, it translocates to the mitochondria and instigates apoptosis.

Long Name

BCL2/Adenovirus E1B 19 Kda Protein-Interacting Protein 3-Like

Alternate Names

Nip3

Entrez Gene IDs

664 (Human)

Gene Symbol

BNIP3

UniProt

Q12983

Additional BNIP3 Products

Product Documents for BNIP3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BNIP3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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