BST2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27154
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Primate
Cited:
Human, Mouse
Applications
Validated:
Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A portion of amino acids 80-130 of human BST2 was used as the immunogen for this antibody.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BST2 Antibody
Western Blot: BST2 Antibody [NBP2-27154]
Western Blot: BST2 Antibody [NBP2-27154] - Analysis using BST2 antibody. Human heart in the 1) absence and 2) presence of immunizing peptide, 3) human Jurkat, 4) mouse RAW lysate and 5) recombinant human BST2 protein probed with BST2 antibody at 4 ug/mL. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.Immunohistochemistry-Frozen: BST2 Antibody [NBP2-27154]
Immunohistochemistry-Frozen: BST2 Antibody [NBP2-27154] - BST-2 (green) was detected in human skin (nevus) using Ang-2 antibody (1:50; 1 hour) and secondary anti-rabbit-FITC (1:500; 30 min) in PBS. Nuclei were stained with DAPI (blue). Tissue was fixed in acetone. Image from a verified customer review.Immunohistochemistry-Paraffin: BST2 Antibody [NBP2-27154]
Immunohistochemistry-Paraffin: BST2 Antibody [NBP2-27154] - Analysis of BST2 in human liver tissue using BST2 antibody at 5 ug/mL.Applications for BST2 Antibody
Application
Recommended Usage
Flow (Cell Surface)
reported in scientific literature (PMID 21695198)
Immunohistochemistry-Frozen
reported by customer review
Immunohistochemistry-Paraffin
5 ug/mL
Simple Western
1:200
Western Blot
2 - 4 ug/mL
Application Notes
BST2 is a 180 amino acid protein with an observed molecular weight of approximately 30 kDa in Western blot (see Ishikawa et all, 1995 PMID 7607676).
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 3 reviews rated 4 using NBP2-27154 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.1 mL PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BST-2/Tetherin
Long Name
Bone Marrow Stromal Cell Antigen 2
Alternate Names
BST2, CD317, HM1.24 Antigen, NPC-A-7, Tetherin
Gene Symbol
BST2
UniProt
Additional BST-2/Tetherin Products
Product Documents for BST2 Antibody
Product Specific Notices for BST2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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