BST2 Antibody - Azide Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45265

Novus Biologicals, part of Bio-Techne
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NBP2-45265
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Predicted:

Chimpanzee (100%), Orangutan (100%), Rhesus Macaque (94%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free

Concentration

1.0 mg/ml

View all BST-2/Tetherin Antibodies »

Product Specifications

Immunogen

A portion of amino acids 80-130 of human BST2 was used as the immunogen for this antibody.

Reactivity Notes

The BST2 amino acid sequence used as immunogen for this antibody is 100% homologous in White-cheeked Gibbon, Great Ape, and Gibbon.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BST2 Antibody - Azide Free

Western Blot: BST2 AntibodyAzide Free [NBP2-45265]

Western Blot: BST2 AntibodyAzide Free [NBP2-45265]

Western Blot: BST2 Antibody - Azide Free [NBP2-45265] - Analysis using BST2 antibody. Human heart in the 1) absence and 2) presence of immunizing peptide, 3) human Jurkat, 4) mouse RAW lysate and 5) recombinant human BST2 protein probed with BST2 antibody at 4 ug/mL. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Image using the standard format of this product.
Immunohistochemistry: BST2 Antibody - Azide Free [NBP2-45265]

Immunohistochemistry: BST2 Antibody - Azide Free [NBP2-45265]

Immunohistochemistry: BST2 Antibody - Azide Free [NBP2-45265] - Analysis of BST2 in human liver tissue using BST2 antibody at 5 ug/mL. Image using the standard format of this product.
Immunohistochemistry: BST2 Antibody - Azide Free [NBP2-45265]

Immunohistochemistry: BST2 Antibody - Azide Free [NBP2-45265]

Immunohistochemistry: BST2 Antibody - Azide Free [NBP2-45265] - Mouse femur bone marrow section stained with BST2 antibody (red) and DAPI (blue). Image from a verified customer review. Image using the standard format of this product.
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Applications for BST2 Antibody - Azide Free

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (Crother et al (2011); PMID 21695198)

Immunohistochemistry-Paraffin

5 ug/ml

Simple Western

1:200

Western Blot

2-4 ug/ml
Application Notes
BST2 is a 180 amino acid protein with an observed molecular weight of approximately 30 kDa in western blotting (see Ishikawa et all, 1995 PMID 7607676). In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BST-2/Tetherin

BST2, also known as CD317, HM1.24 antigen, DAMP-2 and recently designated tetherin, is an integral membrane protein with a novel topology. The specific function of the BST2, encoded by the bone marrow stromal cell antigen 2 gene, is undetermined; however, it may play a role in pre-B-cell growth, rheumatoid arthritis, and in antiretroviral defense, that blocks release of retrovirus from the cell surface. BST2 is highly expressed during B-cell development, from pro-B precursors to plasma cells, T-cells, monocytes, Nk cells and DCs (at protein level).

Long Name

Bone Marrow Stromal Cell Antigen 2

Alternate Names

BST2, CD317, HM1.24 Antigen, NPC-A-7, Tetherin

Gene Symbol

BST2

Additional BST-2/Tetherin Products

Product Documents for BST2 Antibody - Azide Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BST2 Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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