c-jun Antibody (OTI3G2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02258

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3G2

Concentration

0.9 mg/ml

View all c-Jun Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human JUN (NP_002219) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

35.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for c-jun Antibody (OTI3G2)

Western Blot: c-jun Antibody (OTI3G2) [NBP2-02258]

Western Blot: c-jun Antibody (OTI3G2) [NBP2-02258]

Western Blot: Jun Antibody (3G2) [NBP2-02258] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Jun (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Jun.
Immunocytochemistry/Immunofluorescence: Jun Antibody (3G2) [NBP2-02258] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Jun.
Flow Cytometry: c-jun Antibody (OTI3G2) [NBP2-02258]

Flow Cytometry: c-jun Antibody (OTI3G2) [NBP2-02258]

Flow Cytometry: Jun Antibody (3G2) [NBP2-02258] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Jun antibody, and then analyzed by flow cytometry.
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Applications for c-jun Antibody (OTI3G2)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.9 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: c-Jun

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq].

Long Name

Cellular Repressor of E1A-stimulated Genes/Transcription Factor AP-1

Alternate Names

cJun, JUN

Entrez Gene IDs

3725 (Human)

Gene Symbol

JUN

Additional c-Jun Products

Product Documents for c-jun Antibody (OTI3G2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for c-jun Antibody (OTI3G2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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