c-jun Antibody (OTI3G2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71059

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3G2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all c-Jun Antibodies »

Product Summary for c-jun Antibody (OTI3G2) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human JUN (NP_002219) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

35.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for c-jun Antibody (OTI3G2) - Azide and BSA Free

Western Blot: c-jun Antibody (OTI3G2)Azide and BSA Free [NBP2-71059]

Western Blot: c-jun Antibody (OTI3G2)Azide and BSA Free [NBP2-71059]

Western Blot: c-jun Antibody (OTI3G2) - Azide and BSA Free [NBP2-71059] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Jun (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Jun.
Immunocytochemistry/ Immunofluorescence: c-jun Antibody (OTI3G2) - Azide and BSA Free [NBP2-71059]

Immunocytochemistry/ Immunofluorescence: c-jun Antibody (OTI3G2) - Azide and BSA Free [NBP2-71059]

Immunocytochemistry/Immunofluorescence: c-jun Antibody (OTI3G2) - Azide and BSA Free [NBP2-71059] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Jun.
Flow Cytometry: c-jun Antibody (OTI3G2) - Azide and BSA Free [NBP2-71059]

Flow Cytometry: c-jun Antibody (OTI3G2) - Azide and BSA Free [NBP2-71059]

Flow Cytometry: c-jun Antibody (OTI3G2) - Azide and BSA Free [NBP2-71059] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Jun antibody, and then analyzed by flow cytometry.
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Applications for c-jun Antibody (OTI3G2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: c-Jun

The human protooncogene JUN is the putative transforming gene of avian sarcoma virus 17, and it encodes a protein which is highly homologous to the viral protein. cJun (previously known as the Fos binding protein p39) and c Fos form a complex in the nucleus. AP 1 (activating protein 1) is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF subunits that bind to a common DNA site, the AP1 binding site. AP 1 proteins, mostly the Jun group, regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21 (cip1/waf1), p19 (ARF) and p16. Fos and Jun proto oncogene expression is induced transiently by a variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons. JUN has been mapped to 1p32 to p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Long Name

Cellular Repressor of E1A-stimulated Genes/Transcription Factor AP-1

Alternate Names

cJun, JUN

Gene Symbol

JUN

Additional c-Jun Products

Product Documents for c-jun Antibody (OTI3G2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for c-jun Antibody (OTI3G2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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