Key Product Details

Species Reactivity

Validated:

Canine

Cited:

Canine

Applications

Validated:

CyTOF-ready, Flow Cytometry, Western Blot

Cited:

Flow Cytometry, Functional Assay, Surface Plasmon Resonance, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1H6

View all CD34 Antibodies »

Product Specifications

Immunogen

COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant canine CD34

Specificity

Detects canine CD34 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Canine CD34 Antibody

Detection of CD34 antibody in CLL-1390 Canine Cell Line antibody by Flow Cytometry.

Detection of CD34 in CLL‑1390 Canine Cell Line by Flow Cytometry.

CLL-1390 canine leukocytic round cell neoplasia cell line was stained with Mouse Anti-Canine CD34 Monoclonal Antibody (Catalog # MAB3346, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Canine CD34 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: CLL-1390 canine leukocytic round cell neoplasia cell line

Western Blot

McSweeney, P.A. et al. (1998) Blood 91(6):1977. This application was not tested by R&D Systems.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD34

CD34 is a sialomucin molecule that is expressed on primitive hematopoietic stem cells and downregulated as they differentiate into mature cells. Although its precise function remains unknown, the pattern of expression of CD34 suggests that it plays a significant role in early hematopoiesis.

Alternate Names

CD34, HPCA1

Entrez Gene IDs

947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)

Gene Symbol

CD34

Additional CD34 Products

Product Documents for Canine CD34 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Canine CD34 Antibody

For research use only

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