Canine CD8 alpha Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6709

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
MAB6709
MAB6709-SP
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Canine

Cited:

Canine

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 569126

View all CD8 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant canine CD8 alpha
Ser22-Glu186
Accession # P33706

Specificity

Detects canine CD8 alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant CD8 from human, mouse, cotton rat, cat, or guinea pig is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Canine CD8 alpha Antibody

CD8a antibody in Canine PBMCs by Immunocytochemistry (ICC).

CD8 alpha in Canine PBMCs.

CD8a was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Canine CD8a alpha Monoclonal Antibody (Catalog # MAB6709) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Canine CD8 alpha Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed canine peripheral blood mononuclear cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB6709 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD8

CD8 is a dimeric complex made up of two Ig superfamily members. The CD8 alpha chain is a 34 kDa type I transmembrane glycoprotein that is disulfide-linked either to itself forming CD8 alpha alpha, or to an unrelated 30‑35 kDa CD8 beta chain, forming CD8 alpha beta. CD8 alpha contains one V-type Ig-like domain in its extracellular region (ECD) that binds to class I MHC molecules. CD8 alpha beta is a TCR coreceptor, while CD8 alpha alpha promotes T cell survival and differentation. Within the ECD, canine CD8 alpha shares 63%, 45%, 43%, 68%, and 54% amino acid identity with human, mouse, rat, feline, and porcine CD8 alpha, respectively.

Alternate Names

CD8, CD8A

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Gene Symbol

CD8A

UniProt

P33706

Additional CD8 Products

Product Documents for Canine CD8 alpha Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Canine CD8 alpha Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.