Canine/Equine CD44 Antibody

Detection of CD44 in Canine Monocytes by Flow Cytometry.

Canine blood-derived monocytes were stained with Mouse Anti-Canine/Equine CD44 Monoclonal Antibody (Catalog # MAB5449, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B).

Detection of CD44 in Equine PBMCs by Flow Cytometry.

Equine peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Canine/Equine CD44 Monoclonal Antibody (Catalog # MAB5449, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Detection of Equine CD44 by Flow Cytometry

Immunophenotyping and multipotency assay. Representative dot plots from flow cytometry analysis (A). Expression of surface antigens were investigated in cells cultured in control and AZA/RES supplemented medium. Isolated cells were characterized by the expression of CD90 and CD44 while lacked the expression of CD45 hematopoietic marker (B). Multipotency of isolated cells was confirmed by three lineage differentiation (C). In order to confirm formation of proteoglycans during chondrogenesis cells were stained with Safranin O. Intracellular lipid droplets were visualized by Oil Red O while mineralized matrix formed during osteogenesis with Alizarin Red. Magnification ×100, scale bars: 250 μm. Results expressed as mean ± SD. Statistical significance indicated as asterisk (*) when comparing the result to ASCCTRL, and as hashtag (#) when comparing to ASCEMS. ###P < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30370650), licensed under a CC-BY license. Not internally tested by R&D Systems.