Carbonic Anhydrase I/CA1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB120-6619
![Western Blot: Carbonic Anhydrase I/CA1 Antibody [NB120-6619]](https://resources.bio-techne.com/images/products/Carbonic-Anhydrase-I-CA1-Antibody-Western-Blot-NB120-6619-img0004.jpg "Western Blot: Carbonic Anhydrase I/CA1 Antibody [NB120-6619]")
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Carbonic Anhydrase I/CA1/CA1 [Human Erythrocytes] (Uniprot: P00915)
Reactivity Notes
Cross reactivity against Carbonic Anhydrase I/CA1 from other tissues and species may occur but have not been specifically determined.
Localization
Cytoplasmic
Specificity
Cross reactivity against Carbonic Anhydrase I/CA1 from other tissues and species may occur but have not been specifically determined.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Carbonic Anhydrase I/CA1 Antibody
Western Blot: Carbonic Anhydrase I/CA1 Antibody [NB120-6619]
Western Blot: Carbonic Anhydrase I/CA1 Antibody [NB120-6619] - Lane 1: Carbonic Anhydrase I. Load: 50 ng per lane. Primary antibody: Carbonic Anhydrase I antibody at 1:1,000 overnight at 4C. Secondary antibody: HRP goat secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase I.![Immunohistochemistry: Carbonic Anhydrase I/CA1 Antibody [NB120-6619]](https://resources.bio-techne.com/images/products/Carbonic-Anhydrase-I-CA1-Antibody-Immunohistochemistry-NB120-6619-img0001.jpg "Immunohistochemistry: Carbonic Anhydrase I/CA1 Antibody [NB120-6619]")
Immunohistochemistry: Carbonic Anhydrase I/CA1 Antibody [NB120-6619]
Immunohistochemistry: Carbonic Anhydrase I/CA1 Antibody [NB120-6619] - Tissue: spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Incubated at 5 ug/mL for 1 h at RT.
Carbonic Anhydrase I/CA1 Antibody
Immunohistochemistry of Carbonic Anhydrase I/CA1 Antibody. Tissue: human spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-Carbonic Anhydrase I antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase goat secondary antibody at 1:10,000 for 45 min at RT. Staining: Carbonic Anhydrase 1 as precipitated red signal with hematoxylin purple nuclear counterstain.Applications for Carbonic Anhydrase I/CA1 Antibody
Application
Recommended Usage
ELISA
1:2000-1:20000
Immunohistochemistry
10ug/ml
Western Blot
1:500-1:2000
Application Notes
This product has been tested in western blot and immunohistochemistry and is suitable in ELISA. Specific conditions for reactivity should be optimized by the end user.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Carbonic Anhydrase I/CA1
Additional Carbonic Anhydrase I/CA1 Products
Product Documents for Carbonic Anhydrase I/CA1 Antibody
Product Specific Notices for Carbonic Anhydrase I/CA1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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