Carbonic Anhydrase II/CA2 Antibody [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NB600-619
Key Product Details
Species Reactivity
Validated:
Human, Bovine
Cited:
Human
Applications
Validated:
Dot Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
HRP
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carbonic Anhydrase II/CA2/CA2 [Bovine Erythrocytes] (Uniprot: P00921)
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 9336012).
Localization
Cytoplasmic
Specificity
Cross reactivity against Carbonic Anhydrase II/CA2 from other tissues and species may occur but have not been specifically determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This vial contains a relatively low volume of reagent (25 uL). To minimize loss of volume dilute 1:10 by adding 225 uL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20C or below after dilution. Avoid cycles of freezing and thawing.
Carbonic anhydrase II is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes]
Carbonic anhydrase II is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes]
Scientific Data Images for Carbonic Anhydrase II/CA2 Antibody [HRP]
Dot Blot: Carbonic Anhydrase II/CA2 Antibody [HRP] [NB600-619] - Dot Blot of Rabbit Carbonic Anhydrase II/CA2/CA2 antibody [HRP].Lane 1: 100ng.Lane 2: 33.3ng.Lane 3: 11.1ng.Lane 4: 3.7ng.Lane 5: 1.23ng.Secondary Antibody: 200-4357 Anti-Carbonic Anhydrase II/CA2 HRP 1ug/mL.Blocking Buffer for 30 min at RT.
ELISA: Carbonic Anhydrase II/CA2 Antibody [HRP] [NB600-619] - ELISA results of purified Carbonic Anhydrase II/CA2/CA2 antibody [HRP] tested against Carbonic Anhydrase II/CA2 (erythrocytes). (Green line) The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate.
![Carbonic Anhydrase II/CA2 Antibody [HRP]](https://resources.bio-techne.com/images/products/nb600-619_rabbit-polyclonal-carbonic-anhydrase-ii-ca2-antibody-hrp-23520231753180.jpg "Carbonic Anhydrase II/CA2 Antibody [HRP]")
Carbonic Anhydrase II/CA2 Antibody [HRP]
Dot Blot of Rabbit Anti-Carbonic Anhydrase II Peroxidase Conjugated Antibody. Lane 1: 100ng. Lane 2: 33.3ng. Lane 3: 11.1ng. Lane 4: 3.7ng. Lane 5: 1.23ng. Secondary Antibody: 200-4357 Anti-Carbonic Anhydrase II HRP 1ug/mL. Blocking Buffer: for 30 min at RT.Applications for Carbonic Anhydrase II/CA2 Antibody [HRP]
Application
Recommended Usage
ELISA
1:5000-1:20000
Immunohistochemistry
1:500-1:2500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:1000-1:5000
Application Notes
This product has been tested by ELISA and dot blot and is assayed against 1.0 ug of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product.
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C in the dark. Avoid freeze-thaw cycles.
Background: Carbonic Anhydrase II/CA2
Additional Carbonic Anhydrase II/CA2 Products
Product Documents for Carbonic Anhydrase II/CA2 Antibody [HRP]
Product Specific Notices for Carbonic Anhydrase II/CA2 Antibody [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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