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Carbonic Anhydrase II/CA2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-943

Novus Biologicals, part of Bio-Techne
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NB600-943-100ug

Key Product Details

Species Reactivity

Validated:

Human, Bovine

Cited:

Human

Applications

Validated:

ELISA, Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Carbonic Anhydrase II/CA2/CA2 [Bovine Erythrocytes] (Uniprot: P00921)

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 9336012).

Localization

Cytoplasmic

Specificity

Cross reactivity against Carbonic Anhydrase II/CA2 from other tissues and species may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Carbonic Anhydrase II/CA2 Antibody

Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-943]

Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-943]

Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-943] - Lane 1: Carbonic Anhydrase II. Lane 2: None. Load: 50 ng per lane. Primary antibody: Carbonic AnhydraseII primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Alkaline Phosphatase. Other band(s): None.
Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody [NB600-943]

Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody [NB600-943]

Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody [NB600-943] - Tissue: intestine Staining: diffuse positivity of enzyma Carbonic Anidrase II in brush border of villus.
Carbonic Anhydrase II/CA2 Antibody

Carbonic Anhydrase II/CA2 Antibody

Immunohistochemistry of Carbonic Anhydrase II/CA2 Antibody Tissue: intestine Staining: diffuse positivity of enzyme Carbonic Anhydrase II in brush border of villus

Applications for Carbonic Anhydrase II/CA2 Antibody

Application
Recommended Usage

ELISA

1:20000-1:100000

Western Blot

1:2000-1:10000
Application Notes
This product has been tested by Western blot and immunohistochemistry. This product is suitable to be assayed against 1.0 ug of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and TMBE as a substrate for 30 minutes at room temperature.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase II/CA2

Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29 kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20

Alternate Names

CA2

Gene Symbol

CA2

UniProt

Additional Carbonic Anhydrase II/CA2 Products

Product Documents for Carbonic Anhydrase II/CA2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Carbonic Anhydrase II/CA2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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