Carbonic Anhydrase II/CA2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-943
Key Product Details
Species Reactivity
Validated:
Human, Bovine
Cited:
Human
Applications
Validated:
ELISA, Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Carbonic Anhydrase II/CA2/CA2 [Bovine Erythrocytes] (Uniprot: P00921)
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 9336012).
Localization
Cytoplasmic
Specificity
Cross reactivity against Carbonic Anhydrase II/CA2 from other tissues and species may occur but have not been specifically determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Carbonic Anhydrase II/CA2 Antibody
Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-943]
Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-943] - Lane 1: Carbonic Anhydrase II. Lane 2: None. Load: 50 ng per lane. Primary antibody: Carbonic AnhydraseII primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Alkaline Phosphatase. Other band(s): None.Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody [NB600-943]
Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody [NB600-943] - Tissue: intestine Staining: diffuse positivity of enzyma Carbonic Anidrase II in brush border of villus.Carbonic Anhydrase II/CA2 Antibody
Immunohistochemistry of Carbonic Anhydrase II/CA2 Antibody Tissue: intestine Staining: diffuse positivity of enzyme Carbonic Anhydrase II in brush border of villusApplications for Carbonic Anhydrase II/CA2 Antibody
Application
Recommended Usage
ELISA
1:20000-1:100000
Western Blot
1:2000-1:10000
Application Notes
This product has been tested by Western blot and immunohistochemistry. This product is suitable to be assayed against 1.0 ug of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and TMBE as a substrate for 30 minutes at room temperature.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Carbonic Anhydrase II/CA2
Additional Carbonic Anhydrase II/CA2 Products
Product Documents for Carbonic Anhydrase II/CA2 Antibody
Product Specific Notices for Carbonic Anhydrase II/CA2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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