Carbonic Anhydrase XII/CA12 Antibody (CL0280)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52902

Novus Biologicals, part of Bio-Techne
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NBP2-52902
NBP2-52902-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0280

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Carbonic Anhydrase XII/CA12 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: TASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQ

Epitope

HLQHVKYKGQEAFVP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Carbonic Anhydrase XII/CA12 Antibody (CL0280)

Western Blot: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902]

Western Blot: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902]

Western Blot: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902] - Lane 1: Marker [kDa], Lane 2: Human RT-4 cell line
Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902] - Staining of human pancreas shows strong membranous and moderate cytopalsmic positivity in the exocrine cells.
Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902] - Staining of human colon shows strong cytoplasmic and membrane positivity in glandular cells.
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Applications for Carbonic Anhydrase XII/CA12 Antibody (CL0280)

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase XII/CA12

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene.

Alternate Names

CA12

Gene Symbol

CA12

Additional Carbonic Anhydrase XII/CA12 Products

Product Documents for Carbonic Anhydrase XII/CA12 Antibody (CL0280)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Carbonic Anhydrase XII/CA12 Antibody (CL0280)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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