Staining of human kidney shows strong membranous and/or moderate cytoplasmic immunoreactivity in subsets of renal tubules, while glomeruli are negative.

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Applications for Carbonic Anhydrase XII/CA12 Antibody (CL0280) - Azide and BSA Free

Application

Recommended Usage

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase XII/CA12

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene.

Alternate Names

CA12

Gene Symbol

CA12

Additional Carbonic Anhydrase XII/CA12 Products

Product Documents for Carbonic Anhydrase XII/CA12 Antibody (CL0280) - Azide and BSA Free

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for Carbonic Anhydrase XII/CA12 Antibody (CL0280) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Carbonic Anhydrase XII/CA12 Antibody (CL0280) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44059

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Format

Concentration

View all Carbonic Anhydrase XII/CA12 Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Scientific Data Images for Carbonic Anhydrase XII/CA12 Antibody (CL0280) - Azide and BSA Free

Western Blot: Carbonic Anhydrase XII/CA12 Antibody [NBP3-44059]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody [NBP3-44059]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody [NBP3-44059]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody [NBP3-44059]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody [NBP3-44059]

Applications for Carbonic Anhydrase XII/CA12 Antibody (CL0280) - Azide and BSA Free

Immunohistochemistry-Paraffin

Western Blot

Formulation, Preparation, and Storage

Purification

Reconstitution

Formulation

Format

Concentration

Shipping

Stability & Storage

Background: Carbonic Anhydrase XII/CA12

Alternate Names

Gene Symbol

Additional Carbonic Anhydrase XII/CA12 Products

Product Documents for Carbonic Anhydrase XII/CA12 Antibody (CL0280) - Azide and BSA Free

Product Datasheets

COA

Certificate of Analysis

Product Specific Notices for Carbonic Anhydrase XII/CA12 Antibody (CL0280) - Azide and BSA Free