CARD8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56181

Novus Biologicals, part of Bio-Techne
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NB100-56181
NB100-56181SS
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all CARD8 Antibodies »

Product Specifications

Immunogen

Full-length recombinant human TUCAN (CARD8) protein was used as immunogen. The recombinant protein sequence corresponds to GenBank no. gi|14424229|sp|Q9Y2G2|CARD8_HUMAN.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CARD8 Antibody

Western Blot: CARD8 Antibody [NB100-56181]

Western Blot: CARD8 Antibody [NB100-56181]

Western Blot: CARD8 Antibody [NB100-56181] - Analysis of CARD8 in tumor cell lines using this antibody. Cell lysates were normalized for total protein content.
Immunohistochemistry-Paraffin: CARD8 Antibody [NB100-56181]

Immunohistochemistry-Paraffin: CARD8 Antibody [NB100-56181]

Immunohistochemistry-Paraffin: CARD8 Antibody [NB100-56181] - Formalin-fixed, paraffin-embedded tumor/normal adjacent tissue cores from a human colon tissue microarray stained for TUCAN/CARD8 expression at 1:2000 using NB100-56181. A and B are tumor tissue cores. A1 and B1 are the matched normal adjacent cores from A and B, respectively. Hematoxylin-eosin counterstain.
Immunohistochemistry-Paraffin: CARD8 Antibody [NB100-56181]

Immunohistochemistry-Paraffin: CARD8 Antibody [NB100-56181]

Immunohistochemistry-Paraffin: CARD8 Antibody [NB100-56181] - Formalin-fixed, paraffin-embedded tissue human colon microarray stained for TUCAN/CARD8 expression at 1:2000 using NB100-56181. A-A2, sucessively higher magnifications of the microarray. A, low magnification overview. A1, differential TUCAN/CARD8 staining between tissue cores is observed. A2, Areas of intense TUCAN/CARD8 staining in a colon carcinoma tissue core. Hematoxylin eosin counterstain.

Applications for CARD8 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000
Application Notes
The gene encoding TUCAN produces, by alternative splicing, multiple transcripts, with introns, putatively encoding numerous different protein isoforms. Thus the molecular weight observed by SDS-PAGE can vary depending on the TUCAN form expressed.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CARD8

TUCAN (also known as CARD8 and CARDINAL) is a CARD domain containing protein. TUCAN stands for tumor-up-regulated CARD-containing antagonist of caspase nine. Proteins containing a CARD (caspase-associated recruitment domain) domain are key regulators of cell death, cell survival and cytokine production (reviewed in Damiano and Reed, 2004). CARD domains are found in the N-terminal pro-domains of certain caspases, a family of apoptotic and pro-inflammatory proteases, as well as in a diversity of other proteins including TUCAN. CARD domains are homotypic protein interaction motifs that enable networks of proteins to communicate via CARD-CARD interactions. TUCAN is an anti-apoptotic CARD protein that can protect tumors from cell death stimuli, and is overexpressed in certain forms of cancer. The role of TUCAN in tumor biology remains to be fully elucidated, and there appears to be be various mechanisms by TUCAN can potentially contribute to apoptosis resistance (reviewed in Checinska et al, 2006). For example, TUCAN has been shown to inhibit caspase-9 activation by binding to the CARD region of pro-caspase-9, thereby suppressing the formation of the Apaf-1-caspase-9 apoptotic complex and apoptosis. Additionally, a TUCAN isoform has been described that blocks both caspase-8 and caspase-9 mediated apoptosis. However, in some tumors, TUCAN does not appear to interact with caspase-9 and may play a role in modulating NF-kB transcription factor survivial signaling pathways. In this regard a CARD-independent interaction of TUCAN with Ikkg has been described, resulting in the inhibition of interleukin-1 and TNF-induced NF-kB activation. Human TUCAN is a 431 amino acid protein according to GenBank no. gi|14424229|sp|Q9Y2G2|CARD8_HUMAN, and migrates at ~45-49 kDa on SDS-PAGE.

Alternate Names

Apoptotic protein NDPP1, CARD inhibitor of NF-kappaB-activating ligands, CARDINALFLJ18121, CARD-inhibitor of NF-kappa-B-activating ligand, caspase recruitment domain family, member 8, caspase recruitment domain-containing protein 8, DACAR, DAKAR, KIAA0955DKFZp779L0366, NDPP, NDPP1MGC57162, TUCANFLJ18119, Tumor up-regulated CARD-containing antagonist of CASP9, tumor up-regulated CARD-containing antagonist of caspase nine

Entrez Gene IDs

22900 (Human)

Gene Symbol

CARD8

Additional CARD8 Products

Product Documents for CARD8 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CARD8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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