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Caspase-8 Antibody (1G12) [DyLight 594]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80097DL594

Novus Biologicals, part of Bio-Techne
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NBP2-80097DL594

Key Product Details

Species Reactivity

Validated:

Mouse

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Western Blot

Label

DyLight 594 (Excitation = 593 nm, Emission = 618 nm)

Antibody Source

Monoclonal Rat IgG1 kappa Clone # 1G12

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Caspase-8 Antibody (1G12) [DyLight 594]

Immunogen

Recombinant mouse caspase-8 (p20 subunit).

Specificity

Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with human caspase-8.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1 kappa

Applications for Caspase-8 Antibody (1G12) [DyLight 594]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Caspase-8

Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family that is involved in the execution-phase of cell apoptosis. The sequential activation of caspases plays a central role in the execution-phase of cell apoptosis, and Caspase 8 is the most upstream protease in the the caspase activation cascade.

Caspase 8 is expressed as an inactive proenzyme which then undergos proteolytic processing by upstream caspases, to form an active (cleaved) caspase 8 form.

Caspase 8 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.

Alternate Names

CASP8, Caspase8, Mch5

Gene Symbol

CASP8

Additional Caspase-8 Products

Product Documents for Caspase-8 Antibody (1G12) [DyLight 594]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Caspase-8 Antibody (1G12) [DyLight 594]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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