Caspase-9 Antibody (SZ29-01) - (Pro and Active)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67362
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SZ29-01
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human Caspase-9 aa 289-338 / 416. (SwissProt: P55211 Human; SwissProt: Q8C3Q9 Mouse)
Localization
Mitochondrion, cytoplasm, cytosol, nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Caspase-9 Antibody (SZ29-01) - (Pro and Active)
Western Blot: Caspase-9 Antibody (SZ29-01)(Pro and Active) [NBP2-67362]
Western Blot: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Western blot analysis of Caspase-9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat AntiImmunocytochemistry/ Immunofluorescence: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362]
Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - ICC staining of Caspase-9 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primarImmunohistochemistry-Paraffin: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362]
Immunohistochemistry-Paraffin: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Analysis of paraffin-embedded mouse spleen tissue using anti-Caspase-9 antibody. Counter stained with hematoxylin.Applications for Caspase-9 Antibody (SZ29-01) - (Pro and Active)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Caspase-9
There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Caspase 3 is considered an initiator, as initiated caspase-9 goes on to cleave the effector procaspase-3 and procaspase-7.
Caspase 9 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.
Alternate Names
CASP9, Caspase9
Gene Symbol
CASP9
Additional Caspase-9 Products
Product Documents for Caspase-9 Antibody (SZ29-01) - (Pro and Active)
Product Specific Notices for Caspase-9 Antibody (SZ29-01) - (Pro and Active)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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