Cathepsin D Antibody (102)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90432
Recombinant Monoclonal Antibody
![Western Blot: Cathepsin D Antibody (102) [NBP2-90432]](https://resources.bio-techne.com/images/products/Cathepsin-D-Antibody-102-Western-Blot-NBP2-90432-img0002.jpg "Western Blot: Cathepsin D Antibody (102) [NBP2-90432]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 102
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Cathepsin D Antibody (102)
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Cathepsin D (Accession#: NP_034113.1; Met 1-Leu 410).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Cathepsin D Antibody (102)
Western Blot: Cathepsin D Antibody (102) [NBP2-90432]
Western Blot: Cathepsin D Antibody (102) [NBP2-90432] - Anti-Cathepsin D rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 45 kDa (We are unsure as to the identity of these extra bands).![Immunoprecipitation: Cathepsin D Antibody (102) [NBP2-90432]](https://resources.bio-techne.com/images/products/Cathepsin-D-Antibody-102-Immunoprecipitation-NBP2-90432-img0001.jpg "Immunoprecipitation: Cathepsin D Antibody (102) [NBP2-90432]")
Immunoprecipitation: Cathepsin D Antibody (102) [NBP2-90432]
Immunoprecipitation: Cathepsin D Antibody (102) [NBP2-90432] - Mouse Cathepsin D was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 0.5 ul anti-Mouse Cathepsin D rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Cathepsin D rabbit monoclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 45 kDaApplications for Cathepsin D Antibody (102)
Application
Recommended Usage
ELISA
1:25000-1:50000
Immunoprecipitation
1-2 uL/mg of lysate
Western Blot
1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cathepsin D
Alternate Names
CTSD
Gene Symbol
CTSD
Additional Cathepsin D Products
Product Documents for Cathepsin D Antibody (102)
Product Specific Notices for Cathepsin D Antibody (102)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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