CCRL2/CRAM-A/B Antibody (BZ5B8) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11970

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-11970-0.025mg
NBP3-11970-0.2mg
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Key Product Details

Species Reactivity

Mouse

Applications

ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # BZ5B8

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CCRL2/CRAM-A/B Antibodies »

Product Specifications

Immunogen

This antibody was raised by immunising Wistar Furth rats with the mCCRL2/CRAM-A/B peptide/KLH conjugate. The immunizing amino-terminal mCCRL2/CRAM-A/B peptide with the sequence NH2-MDNYTVAPDDEYDVLILDDYLDNSC-COOH, corresponding to residues 1-24 of mCCRL2/CRAM-A/B, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).

Specificity

This antibody is specific for murine CCRL2/CRAM-A/B (formerly known as L-CCR), which is expressed on mast cells, activated macrophages, and endothelial cells. This receptor binds the leukocyte chemoattractant chemerin without triggering classical GPCR signaling, providing a specific mechanism for local enrichment of chemerin at inflammatory sites. CCRL2/CRAM-A/B presents active chemerin to leukocytes expressing the chemoattractant receptor CMKLR1/Chemr23. Chemerin can block anti-CCRL2/CRAM-A/B antibody binding.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for CCRL2/CRAM-A/B Antibody (BZ5B8) - Chimeric - Azide and BSA Free

Western Blot: CCRL2/CRAM-A/B Antibody (BZ5B8)ChimericAzide and BSA Free [NBP3-11970]

Western Blot: CCRL2/CRAM-A/B Antibody (BZ5B8)ChimericAzide and BSA Free [NBP3-11970]

Western Blot: CCRL2/CRAM-A/B Antibody (BZ5B8) - Chimeric [NBP3-11970] - Mouse thymus (A) and mouse brain (B) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of CCRL2 (NBP3-11970) at 0.5 ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: CCRL2/CRAM-A/B Antibody (BZ5B8) - Chimeric - Azide and BSA Free [NBP3-11970]

Immunocytochemistry/ Immunofluorescence: CCRL2/CRAM-A/B Antibody (BZ5B8) - Chimeric - Azide and BSA Free [NBP3-11970]

Immunocytochemistry/Immunofluorescence: CCRL2/CRAM-A/B Antibody (BZ5B8) - Chimeric [NBP3-11970] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of BZ5B8 (NBP3-11970) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11970, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Applications for CCRL2/CRAM-A/B Antibody (BZ5B8) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional Assay

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CCRL2/CRAM-A/B

CCRL2 encodes a chemokine receptor like protein, which is predicted to be a seven transmembrane protein and most closely related to CCR1. Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. This gene is expressed at high levels in primary neutrophils and primary monocytes, and is further upregulated on neutrophil activation and during monocyte to macrophage differentiation. The function of this gene is unknown. This gene is mapped to the region where the chemokine receptor gene cluster is located. [provided by RefSeq]

Long Name

CC Chemokine Receptor-like 2

Alternate Names

ACKR5, CCRL2, CKRX, CRAM-A, CRAM-B, CRAMA, CRAMB, HCR

Gene Symbol

CCRL2

Additional CCRL2/CRAM-A/B Products

Product Documents for CCRL2/CRAM-A/B Antibody (BZ5B8) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CCRL2/CRAM-A/B Antibody (BZ5B8) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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