Immunohistochemistry: CD11a/CD18 Antibody (hu1124 (Efalizumab)) - Chimeric [NBP2-52686] - Anti-CD11a staining of formaldehyde fixed paraffin embedded rat spleen tissue, at 40x magnification. The human IgG1-chimeric version of hu1124 (NBP2-75912) was used to stain samples at a concentration of 3 ug/ml.

Flow Cytometry: CD11a/CD18 Antibody (hu1124 (Efalizumab)) - Chimeric - Azide and BSA Free [NBP2-52686]

Flow Cytometry: CD11a/CD18 Antibody (hu1124 (Efalizumab)) - Chimeric [NBP2-52686] - Flow-cytometry using the anti-CD11a research biosimilar antibody Efalizumab. Human lymphocytes were stained with an isotype control (NBP2-52638, panel A) or the rabbit-chimeric version of Efalizumab (NBP2-52686, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Applications

Application

Recommended Usage

Flow Cytometry

1:10 - 1:1000

Application Notes

This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques. Efalizumab binds to the LFA-1 alpha I domain blocking the ICAM-1 binding site via steric hindrance.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD11a/CD18

The CD11/18 complex of glycoproteins are dimers comprised of a common beta chain and individual alpha chains (CD11a/18 or CD11b/18 and CD11c/18). CD11a/18 is a receptor for C3bi, a complement component, CD11b/18 is Lymphocyte Function Activator, and CDD11c/18 is a lectin-like glycoprotein thought to be important for mediation of cells to other surfaces.

Alternate Names

CD11 antigen-like family member A, CD11a antigen, CD11Aantigen CD11A (p180), lymphocyte function-associated antigen 1, alphapolypeptide, integrin alpha-L, integrin gene promoter, integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen1; alpha polypeptide), Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocyte function-associated molecule 1 alpha chain, LFA-1, LFA-1 alpha, LFA1A, LFA-1A, lymphocyte function-associated antigen 1, biosimilar antibody, biosimilars

Gene Symbol

ITGAL

Additional CD11a/CD18 Products

Product Documents

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

CD11a/CD18 Antibody (hu1124 (Efalizumab)) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52686

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all CD11a/CD18 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images

Immunohistochemistry: CD11a/CD18 Antibody (hu1124 (Efalizumab)) - Chimeric - Azide and BSA Free [NBP2-52686]

Flow Cytometry: CD11a/CD18 Antibody (hu1124 (Efalizumab)) - Chimeric - Azide and BSA Free [NBP2-52686]

Applications

Flow Cytometry

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: CD11a/CD18

Alternate Names

Gene Symbol

Additional CD11a/CD18 Products

Product Documents

Product Datasheets

COA

Certificate of Analysis

Product Specific Notices