CD133 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB120-16518

Novus Biologicals, part of Bio-Techne
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NB120-16518SS
NB120-16518
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Cited:

Human, Mouse, Rat, Porcine

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Block/Neutralize, ELISA, Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all CD133 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to a C-terminal region of CD133 (within amino acids 750-865).

Reactivity Notes

porcine reactivity reported in scientific literature (PMID: 29352176).

Specificity

CD133 - Hematopoietic Stem Cell Marker

Marker

Stem Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD133 Antibody - BSA Free

Western Blot: CD133 AntibodyBSA Free [NB120-16518]

Western Blot: CD133 AntibodyBSA Free [NB120-16518]

Western Blot: CD133 Antibody - BSA Free [NB120-16518] - Predicted band size : 97 kDa recognizes 97 kDa human CD133 in Y79 cells. Band is at ~120 kDa due to protein glycosylation.
Immunocytochemistry/ Immunofluorescence: CD133 Antibody - BSA Free [NB120-16518]

Immunocytochemistry/ Immunofluorescence: CD133 Antibody - BSA Free [NB120-16518]

Immunocytochemistry/Immunofluorescence: CD133 Antibody - BSA Free [NB120-16518] - Characterization of Scattered tubular-like cells (STCs) isolated from pig kidneys. A) Representative immunofluorescence staining (original magnification 40X) for the STC surface markers CD133 NB120-16518G (red) and CD24 (green) in isolated swine STCs. B) Flow cytometry analysis of isolated STCs showing that 97% of cells expressed CD133, 95% CD24, and 98% were double-positive for CD133 and CD24 (n = 3 each). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31614781/) licensed under a CC-BY license.
Immunohistochemistry: CD133 Antibody - BSA Free [NB120-16518]

Immunohistochemistry: CD133 Antibody - BSA Free [NB120-16518]

Immunohistochemistry: CD133 Antibody - BSA Free [NB120-16518] - Immunostaining for CD133 (NB120-16518) in normal ovarian and tumor tissue sections. Staining of CD133 in OSE layer (A, B) as well as cortex (C) reveals specific CD133+ cells with relatively higher cell numbers in BL and HG. Area within dotted lines in BN OSE (A) are magnified in (B) while elliptical/spindle shaped CD133+ cells in cortex from various fields were represented in the composite image in (C) of BN and HG. Large CD133+ cells in cortex were also observed. White scale bar?=?50 um; blue scale bar?=?10 um. Secondary antibody employed was conjugated with Alexa fluor 568 and tissue sections were counterstained with nucleus specific dye DAPI. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30121075/) licensed under a CC-BY license.
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Applications for CD133 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100-1:250

Immunohistochemistry-Paraffin

1:100-1:250

Western Blot

1:500-1:1000
Application Notes
Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa). IHC: Citrate buffer antigen retreival required. ICC/IF: Fix with 3% paraformaldehyde for 20 min at RT. Optimal dilutions/concentrations should be determined by the end user. Use in IHC-Frozen reported in scientific literature (PMID: 29352176).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD133

Prominin-1 (also known as CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The Prominin-1 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Alternate Names

AC133, CD133, PROM1, Prominin 1, PROML1

Gene Symbol

PROM1

Additional CD133 Products

Product Documents for CD133 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD133 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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