CD133 Antibody [DyLight 488]
Novus Biologicals, part of Bio-Techne | Catalog # NB120-16518G
![CD133 Antibody [DyLight 488]](https://resources.bio-techne.com/images/products/nb120-16518g_rabbit-polyclonal-cd133-antibody-dylight-488-271220231253383.jpg "Immunocytochemistry/Immunofluorescence: CD133 Antibody [DyLight 488] [NB120-16518G] -")
![CD133 Antibody [DyLight 488]](https://resources.bio-techne.com/images/products/nb120-16518g_rabbit-polyclonal-cd133-antibody-dylight-488-31020241517132.jpg "Immunocytochemistry/ Immunofluorescence: CD133 Antibody [DyLight 488] [NB120-16518G] -")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine
Cited:
Rat
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Flow Cytometry
Label
DyLight 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic peptide corresponding to a C-terminal region of CD133 (within amino acids 750-865).
Reactivity Notes
porcine reactivity reported in scientific literature (PMID: 29352176).
Specificity
CD133 - Hematopoietic Stem Cell Marker
Marker
Stem Cell Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Scientific Data Images for CD133 Antibody [DyLight 488]
Immunocytochemistry/Immunofluorescence: CD133 Antibody [DyLight 488] [NB120-16518G] - Characterization of Scattered tubular-like cells (STCs) isolated from pig kidneys. A) Representative immunofluorescence staining (original magnification 40X) for the STC surface markers CD133 NB120-16518G (red) and CD24 (green) in isolated swine STCs. B) Flow cytometry analysis of isolated STCs showing that 97% of cells expressed CD133, 95% CD24, and 98% were double-positive for CD133 and CD24 (n = 3 each). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31614781/) licensed under a CC-BY license.
Flow Cytometry: CD133 Antibody [DyLight 488] [NB120-16518G] - Percent of EPCs (CD133 + CD34 +) reduced in the XYK group detected by flow cytometry. Data are mean +/-SD analyzed by one-way analysis of variance.**P < 0.01. CSDH, chronic subdural hematoma; EPC, endothelial progenitor cell; XYK, Xiaoyukang Jiaonang. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32328124/) licensed under a CC-BY license.
Flow Cytometry: CD133 Antibody [DyLight 488] [NB120-16518G] - Flow Cytometry: CD133 Antibody (DyLight488) [NB120-16518G] - Rat bone marrow cells were stained with CD133 (1:100) antibody (20 minutes at 4C), fixed, and then analyzed.
Applications for CD133 Antibody [DyLight 488]
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
ELISA: 1/1000. WB: 1/1,000 - 1:2,000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa). IHC: Citrate buffer antigen retreival required. ICC/IF: Fix with 3% paraformaldehyde for 20 min at RT. Optimal dilutions/concentrations should be determined by the end user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using NB120-16518G in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CD133
Alternate Names
AC133, CD133, PROM1, Prominin 1, PROML1
Gene Symbol
PROM1
Additional CD133 Products
Product Documents for CD133 Antibody [DyLight 488]
Product Specific Notices for CD133 Antibody [DyLight 488]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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