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CD24 Antibody (SN3)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-64861

Clone SN3 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB100-64861
NB100-64861-0.025mg
Forumulation
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # SN3

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Glycoproteins purified from human NALM-1 cell line

Reactivity Notes

Predicted cross-reactivities: Chimpanzee

Specificity

Recognizes the human CD24 cell surface antigen, a heavily glycosylated molecule of 35-45kD. CD24 is expressed by granulocytes and B lymphocytes, and by some activated T cells and T cell malignancies.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD24 Antibody (SN3)

Flow Cytometry: CD24 Antibody (SN3) - BSA Free [NB100-64861]

Flow Cytometry: CD24 Antibody (SN3) - BSA Free [NB100-64861]

Flow Cytometry: CD24 Antibody (SN3) - BSA Free [NB100-64861] - SUM159PT cells were stained with CD24 antibody (NB100-64861AF488, 0.5 ug/10^6 cells). Left: unstain; Right: CD24 antibody stain. Image from verified customer review.
Flow Cytometry: CD24 Antibody (SN3) - BSA Free [NB100-64861]

Flow Cytometry: CD24 Antibody (SN3) - BSA Free [NB100-64861]

Flow Cytometry: CD24 Antibody (SN3) [NB100-64861] - Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD24: RPE.

Applications for CD24 Antibody (SN3)

Application
Recommended Usage

Flow Cytometry

1/50-1/100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500
Application Notes
This CD24 antibody is validated for IHC-P from a verified customer review.

Reviewed Applications

Read 1 review rated 3 using NB100-64861 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD24

CD24 (also known as heat stable antigen) is a phosphatidylinositol linked cell surface glycoprotein with a polypeptide core of 30 amino acids and high carbohydrate content. The apparent molecular mass of the two forms of CD24 purified from a mouse lymphoma cell line appear to be in the range of 40-60 and 23-30 kDa. CD24 is expressed at all stages of B cell development and on most thymocytes. The absence of expression from mature T cells is closely associated with their maturation from CD4+ CD8+ CD24+ thymocytes to either CD4+ CD8- CD24- or CD4- CD8+ CD24- T cells. CD24 is also present on mouse granulocytes, monocytes, Langerhans cells and erythrocytes. Its ligand is P selectin (CD62P). CD24 may play a role in regulation of B cell proliferation and differentiation, and it has been proposed that heat stable antigen promotes homotypic adhesion between B lymphocytes. CD24 is also a positive marker of pancreatic cancer.

Alternate Names

CD24, CD24A

Gene Symbol

CD24

UniProt

Additional CD24 Products

Product Documents for CD24 Antibody (SN3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD24 Antibody (SN3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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