CD25/IL-2R alpha Antibody (Basiliximab) [Biotin] - Chimeric

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52661B

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Cynomolgus Monkey, Rhesus Macaque

Applications

Block/Neutralize, CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Biotin

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # Basiliximab

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD25/IL-2R alpha Antibodies »

Product Specifications

Immunogen

Basiliximab was prepared by immunizing BALB/c mice with CTC 16 cells.

Specificity

Basiliximab recognises the human IL-2R and binds to the epitope (116)ERIYHFV(122) within the extracellular domain of the CD25 subunit -binding is abolished when 2 or more residues within the epitope are mutated. This epitope overlaps with the interaction site of CD25 and IL-2. CD25 is found in the high-affinity IL-2R ((alpha)(beta)(gamma)c) -T cells only express CD25 upon activation. IL-2 binding to IL-2R on T cells modulates proliferation and differentiation.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Applications for CD25/IL-2R alpha Antibody (Basiliximab) [Biotin] - Chimeric

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD25/IL-2R alpha

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.(provided by RefSeq)

Long Name

Interleukin 2 Receptor alpha

Alternate Names

CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA

Gene Symbol

IL2RA

Additional CD25/IL-2R alpha Products

Product Documents for CD25/IL-2R alpha Antibody (Basiliximab) [Biotin] - Chimeric

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD25/IL-2R alpha Antibody (Basiliximab) [Biotin] - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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