CD25/IL-2R alpha Antibody (OX-39)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-64761

Novus Biologicals, part of Bio-Techne
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NB100-64761
NB100-64761-0.05mg
NB100-64761-100ug
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Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation

Cited:

Flow Cytometry, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OX-39

Concentration

1.0 mg/ml

View all CD25/IL-2R alpha Antibodies »

Product Specifications

Immunogen

Stimulated Rat T cells

Localization

Cell Membrane

Specificity

NB100-64761 recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a 55kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. *This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) ^(6). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD25/IL-2R alpha Antibody (OX-39)

Immunocytochemistry/ Immunofluorescence: CD25/IL-2R alpha Antibody (OX-39) - BSA Free [NB100-64761]

Immunocytochemistry/ Immunofluorescence: CD25/IL-2R alpha Antibody (OX-39) - BSA Free [NB100-64761]

Immunocytochemistry/Immunofluorescence: CD25/IL-2 R alpha Antibody (OX-39) [NB100-64761] - Rat lymph node cryosection with Mouse anti Rat CD25 antibody , red in A and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power
Immunohistochemistry-Paraffin: CD25/IL-2R alpha Antibody (OX-39) - BSA Free [NB100-64761]

Immunohistochemistry-Paraffin: CD25/IL-2R alpha Antibody (OX-39) - BSA Free [NB100-64761]

Immunohistochemistry-Paraffin: CD25/IL-2 R alpha Antibody (OX-39) [NB100-64761] - Rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power
Flow Cytometry: CD25/IL-2R alpha Antibody (OX-39) - BSA Free [NB100-64761]

Flow Cytometry: CD25/IL-2R alpha Antibody (OX-39) - BSA Free [NB100-64761]

Flow Cytometry: CD25/IL-2 R alpha Antibody (OX-39) [NB100-64761] - Staining of Con A activated rat spleen cells with Mouse anti Rat CD25: Biotin
View 1 more images

Applications for CD25/IL-2R alpha Antibody (OX-39)

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD25/IL-2R alpha

The IL2 receptor (IL2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. It is transiently expressed at a low level during B cell development in bone marrow on the CD45R/B220 low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220 low TdT+ sIg- pro-B/pre B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells. CD25 is expressed at a higher level during very early T cell development in fetal and adult thymus.

Long Name

Interleukin 2 Receptor alpha

Alternate Names

CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA

Entrez Gene IDs

25704 (Rat)

Gene Symbol

IL2RA

UniProt

P26897

Additional CD25/IL-2R alpha Products

Product Documents for CD25/IL-2R alpha Antibody (OX-39)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD25/IL-2R alpha Antibody (OX-39)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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