CD25/IL-2R alpha Antibody (OX-39) [FITC]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-64761F
![CD25/IL-2R alpha Antibody (OX-39) [FITC]](https://resources.bio-techne.com/images/products/nb100-64761f_mouse-cd25-il-2r-alpha-mab-ox-39-fitc-31720238132414.jpg "CD25/IL-2R alpha Antibody (OX-39) [FITC] [NB100-64761F] -")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Rat
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # OX-39
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Stimulated Rat T cells
Reactivity Notes
0
Localization
Cell Membrane
Specificity
NB100-64761 recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a 55kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. *This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) ^(6). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CD25/IL-2R alpha Antibody (OX-39) [FITC]
CD25/IL-2R alpha Antibody (OX-39) [FITC] [NB100-64761F] -
CD25/IL-2R alpha Antibody (OX-39) [FITC] [NB100-64761F] - Vial of FITC conjugated antibody. FITC is optimally excited at 498 nm by the Blue laser (488 nm) and has an emission maximum of 519 nm.Applications for CD25/IL-2R alpha Antibody (OX-39) [FITC]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: CD25/IL-2R alpha
Long Name
Interleukin 2 Receptor alpha
Alternate Names
CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA
Gene Symbol
IL2RA
Additional CD25/IL-2R alpha Products
Product Documents for CD25/IL-2R alpha Antibody (OX-39) [FITC]
Product Specific Notices for CD25/IL-2R alpha Antibody (OX-39) [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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