CD3 epsilon Antibody (B-B12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44879

Novus Biologicals, part of Bio-Techne
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NBP2-44879-0.1mg
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # B-B12

Concentration

0.2 mg/ml

View all CD3 epsilon Antibodies »

Product Specifications

Immunogen

Human T cell leukemia cells

Localization

Cell surface and cytoplasmic

Specificity

Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. CD3 is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. The CD3 complex is closely associated at the lymphocyte cell surface with T cell antigen receptor (TCR) and is involved in transducing antigen-recognition signals into cytoplasm of T cells and in regulating the cell surface expression of the TCR complex.

Marker

T-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP3-11505)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD3 epsilon Antibody (B-B12)

Immunocytochemistry/ Immunofluorescence: CD3 epsilon Antibody (B-B12) [NBP2-44879]

Immunocytochemistry/ Immunofluorescence: CD3 epsilon Antibody (B-B12) [NBP2-44879]

Immunocytochemistry/Immunofluorescence: CD3 epsilon Antibody (B-B12) [NBP2-44879] - Immunofluorescence Analysis of Jurkat cells labeling CD3e with CD3 epsilon Antibody (B-B12) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).
Flow Cytometry: CD3 epsilon Antibody (B-B12) [NBP2-44879]

Flow Cytometry: CD3 epsilon Antibody (B-B12) [NBP2-44879]

Flow Cytometry: CD3 epsilon Antibody (B-B12) [NBP2-44879] - Flow Cytometric Analysis of Jurkat cells. CD3 epsilon antibody (B-B12) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Applications for CD3 epsilon Antibody (B-B12)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD3 epsilon

CD3 epsilon is a subunit of CD3. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Alternate Names

CD3e

Gene Symbol

CD3E

UniProt

P07766

Additional CD3 epsilon Products

Product Documents for CD3 epsilon Antibody (B-B12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD3 epsilon Antibody (B-B12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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