CD3 epsilon Antibody (B-B12) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11505
![Flow Cytometry: CD3 epsilon Antibody (B-B12) - Azide and BSA Free [NBP3-11505]](https://resources.bio-techne.com/images/products/CD3-epsilon-Antibody-B-B12-Azide-and-BSA-Free-Flow-Cytometry-NBP3-11505-img0001.jpg "Flow Cytometry: CD3 epsilon Antibody (B-B12) - Azide and BSA Free [NBP3-11505]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # B-B12
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Human T cell leukemia cells
Localization
Cell surface and cytoplasmic
Specificity
Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. CD3 is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. The CD3 complex is closely associated at the lymphocyte cell surface with T cell antigen receptor (TCR) and is involved in transducing antigen-recognition signals into cytoplasm of T cells and in regulating the cell surface expression of the TCR complex.
Marker
T-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-44879).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD3 epsilon Antibody (B-B12) - Azide and BSA Free
Flow Cytometry: CD3 epsilon Antibody (B-B12) - Azide and BSA Free [NBP3-11505]
Flow Cytometry: CD3 epsilon Antibody (B-B12) - Azide and BSA Free [NBP3-11505] - Flow Cytometric Analysis of Jurkat cells. CD3 epsilon antibody (B-B12) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).Applications for CD3 epsilon Antibody (B-B12) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Application Notes
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CD3 epsilon
Alternate Names
CD3e
Gene Symbol
CD3E
Additional CD3 epsilon Products
Product Documents for CD3 epsilon Antibody (B-B12) - Azide and BSA Free
Product Specific Notices for CD3 epsilon Antibody (B-B12) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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