Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry

Label

Janelia Fluor 646

Antibody Source

Recombinant Monoclonal Mouse IgG2A Clone # 425514

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD53 Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CD53
Met1-Leu219
Accession # P19397

Specificity

Detects human CD53. Stains human CD53 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for CD53 Antibody (425514) [Janelia Fluor® 646]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD53

The antibody reacts with CD53 antigen, a glycoprotein of 32-40 kDa belonging to the tetraspans family (TM4SF). CD53 antigen is exclusivelly expressed on leucocytes (not present on platelets, red blood cells and non-hematopoietic cells). CD53 is important for signal transduction. CD53 cross-linking promotes activation of human B cells and rat macrophages.

Alternate Names

CD53, MOX44, TSPAN25

Gene Symbol

CD53

Additional CD53 Products

Product Documents for CD53 Antibody (425514) [Janelia Fluor® 646]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD53 Antibody (425514) [Janelia Fluor® 646]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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