Human CD53 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4624
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 425514
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human CD53
Met1-Leu219
Accession # P19397
Met1-Leu219
Accession # P19397
Specificity
Detects human CD53. Stains human CD53 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human CD53 Antibody
Detection of CD53 in Human Lymphocytes by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD53 Monoclonal Antibody (Catalog # MAB4624, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.Applications for Human CD53 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood lymphocytes
Sample: Human peripheral blood lymphocytes
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD53
CD53, also known as TSPAN25, is a 35 kDa‑40 kDa cell surface and endosomal membrane glycoprotein in the tetraspanin superfamily. CD53 is widely expressed on hematopoietic cells. Ligation of CD53 promotes cell activation and survival as well as homotypic cell‑cell adhesion. CD53 associates with other tetraspanins, MHC class I and II molecules, and integrin alpha4 beta1. Human CD53 shares 81%‑83% aa sequence identity with mouse and rat CD53.
Alternate Names
CD53, MOX44, TSPAN25
Gene Symbol
CD53
UniProt
Additional CD53 Products
Product Documents for Human CD53 Antibody
Product Specific Notices for Human CD53 Antibody
For research use only
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