Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD53 Monoclonal Antibody (Catalog # MAB4624, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD53 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood lymphocytes

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD53

CD53, also known as TSPAN25, is a 35 kDa‑40 kDa cell surface and endosomal membrane glycoprotein in the tetraspanin superfamily. CD53 is widely expressed on hematopoietic cells. Ligation of CD53 promotes cell activation and survival as well as homotypic cell‑cell adhesion. CD53 associates with other tetraspanins, MHC class I and II molecules, and integrin alpha4 beta1. Human CD53 shares 81%‑83% aa sequence identity with mouse and rat CD53.

Alternate Names

CD53, MOX44, TSPAN25

Entrez Gene IDs

963 (Human); 12508 (Mouse); 24251 (Rat)

Gene Symbol

CD53

UniProt

P19397

Additional CD53 Products

Product Documents for Human CD53 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD53 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CD53 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4624

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all CD53 Antibodies »

Product Summary for Human CD53 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD53 Antibody

Detection of CD53 in Human Lymphocytes by Flow Cytometry.