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CD59 Antibody (8D2B8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37436

Novus Biologicals, part of Bio-Techne
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NBP2-37436
NBP2-37436-0.025ml

Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8D2B8

Format

BSA Free

Concentration

1 mg/ml

Product Summary for CD59 Antibody (8D2B8) - BSA Free

Immunogen

Purified recombinant fragment of human CD59 (AA: 31-111) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

14.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD59 Antibody (8D2B8) - BSA Free

Western Blot: CD59 Antibody (8D2B8)BSA Free [NBP2-37436]

Western Blot: CD59 Antibody (8D2B8)BSA Free [NBP2-37436]

Western Blot: CD59 Antibody (8D2B8) [NBP2-37436] - Western blot analysis using CD59 mAb against HEK293 (1) and CD59 (AA: 31-111)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: CD59 Antibody (8D2B8) - BSA Free [NBP2-37436]

Immunocytochemistry/ Immunofluorescence: CD59 Antibody (8D2B8) - BSA Free [NBP2-37436]

Immunocytochemistry/Immunofluorescence: CD59 Antibody (8D2B8) [NBP2-37436] - Immunofluorescence analysis of Hela cells using CD59 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: CD59 Antibody (8D2B8) - BSA Free [NBP2-37436]

Immunohistochemistry: CD59 Antibody (8D2B8) - BSA Free [NBP2-37436]

Immunohistochemistry: CD59 Antibody (8D2B8) [NBP2-37436] - Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CD59 mouse mAb with DAB staining.

Applications for CD59 Antibody (8D2B8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:50 -1:200

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD59

CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells.

Alternate Names

CD59, HRF20, MACIF, MIC11, Protectin

Gene Symbol

CD59

Additional CD59 Products

Product Documents for CD59 Antibody (8D2B8) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD59 Antibody (8D2B8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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