CD59 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89405

Novus Biologicals, part of Bio-Techne
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NBP1-89405
NBP1-89405-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CD59 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CYNCPNPTADCKTAVNCSSDFDACLITKAGLQVYNKCWKFEHCNFNDVTTRLRENELTYYCCKKDLCNFNEQLENGG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD59 Antibody

Western Blot: CD59 Antibody [NBP1-89405]

Western Blot: CD59 Antibody [NBP1-89405]

Western Blot: CD59 Antibody [NBP1-89405] - Analysis in human cell lines PC-3 and Caco-2 using anti-CD59 antibody. Corresponding CD59 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
Immunocytochemistry/ Immunofluorescence: CD59 Antibody [NBP1-89405]

Immunocytochemistry/ Immunofluorescence: CD59 Antibody [NBP1-89405]

Immunocytochemistry/Immunofluorescence: CD59 Antibody [NBP1-89405] - Staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CD59 Antibody [NBP1-89405]

Immunohistochemistry-Paraffin: CD59 Antibody [NBP1-89405]

Immunohistochemistry-Paraffin: CD59 Antibody [NBP1-89405] - Staining of human urinary bladder shows distinct membranous positivity in urothelial cells.
View 1 more images

Applications for CD59 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD59

CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells.

Alternate Names

CD59, HRF20, MACIF, MIC11, Protectin

Entrez Gene IDs

966 (Human)

Gene Symbol

CD59

UniProt

P13987

Additional CD59 Products

Product Documents for CD59 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD59 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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