CD74 Antibody (PIN.1) [PE]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1985PE

Novus Biologicals, part of Bio-Techne
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NB100-1985PE
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Key Product Details

Species Reactivity

Human, Mouse (Negative)

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # PIN.1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD74 Antibodies »

Product Specifications

Immunogen

Peptide corresponding to residues 12-27 of CD74, human invariant chain (short form).

Epitope

The epitope is in a region of the cytoplasmic tail of human CD74 which is common to all isoforms.

Reactivity Notes

Human. Does not react with mouse.

Localization

Type II membrane protein

Specificity

This detects an ~33-35 kDa protein doublet, corresponding to the apparent molecular mass of the p33 and p35 forms of human CD74.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for CD74 Antibody (PIN.1) [PE]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD74

CD74, also known as the MHC class II associated invariant chain (Ii), is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is produced in molar excess over MHC class II and some of this is expressed by an unknown pathway on the cell surface independent of, or in association with, MHC class II molecules. The half life of CD74 on the cell surface is only 3 to 4 min after which it is internalized. It has been proposed that cell surface CD74 binds MHC class II molecules which have lost their antigenic peptide leading to internalization and reloading with antigenic peptide. The transport route of class II alpha/beta/Ii complexes is regulated selectively by two forms of CD74 (p33 and p35) which are generated by the use of alternative translation initiation sites. CD74 is expressed primarily by antigen presenting cells such as B lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages and monocytes together, with many epithelial cells. CD74 may exist in different isoforms ranging in size from 33 to 41 kDa, depending on genetic splicing.

Alternate Names

CD74, CLIP, DHLAG, HLADG, Ia-gamma, INVG34

Gene Symbol

CD74

Additional CD74 Products

Product Documents for CD74 Antibody (PIN.1) [PE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD74 Antibody (PIN.1) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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