CD74 Antibody (PIN.1) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80657
![Flow Cytometry: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657]](https://resources.bio-techne.com/images/products/CD74-Antibody-PIN-1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80657-img0006.jpg "Flow Cytometry: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657]")
![Western Blot: CD74 Antibody (PIN.1)Azide and BSA Free [NBP2-80657]](https://resources.bio-techne.com/images/products/CD74-Antibody-PIN-1-Azide-and-BSA-Free-Western-Blot-NBP2-80657-img0002.jpg "Western Blot: CD74 Antibody (PIN.1)Azide and BSA Free [NBP2-80657]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse (Negative)
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # PIN.1
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for CD74 Antibody (PIN.1) - Azide and BSA Free
Immunogen
Peptide corresponding to residues 12-27 of CD74, human invariant chain (short form).
Epitope
The epitope is in a region of the cytoplasmic tail of human CD74 which is common to all isoforms.
Reactivity Notes
Human. Does not react with mouse.
Localization
Type II membrane protein
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CD74 Antibody (PIN.1) - Azide and BSA Free
Western Blot: CD74 Antibody (PIN.1)Azide and BSA Free [NBP2-80657]
Western Blot: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657] - Western blot of CD74 from IP N87 lysates mixed with macrophage migration inhibitory factor (MIF). Image from the standard format of this antibody.![Immunocytochemistry: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657]](https://resources.bio-techne.com/images/products/CD74-Antibody-PIN-1-Azide-and-BSA-Free-Immunocytochemistry-NBP2-80657-img0004.jpg "Immunocytochemistry: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657]")
Immunocytochemistry: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657]
Immunocytochemistry: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human renal cell carcinoma using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration. Image from the standard format of this antibody.![Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657]](https://resources.bio-techne.com/images/products/CD74-Antibody-PIN-1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80657-img0005.jpg "Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657]")
Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657]
Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human spleen using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration. Image from the standard format of this antibody.![Western Blot: CD74 Antibody (PIN.1)Azide and BSA Free [NBP2-80657]](https://resources.bio-techne.com/images/products/CD74-Antibody-PIN-1-Azide-and-BSA-Free-Western-Blot-NBP2-80657-img0001.jpg "Western Blot: CD74 Antibody (PIN.1)Azide and BSA Free [NBP2-80657]")
![Immunohistochemistry: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657]](https://resources.bio-techne.com/images/products/CD74-Antibody-PIN-1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80657-img0003.jpg "Immunohistochemistry: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657]")
Applications for CD74 Antibody (PIN.1) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1 ug/million cells
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Immunoprecipitation
1:10-1:500
Western Blot
1 ug/ml
Application Notes
In Simple Western only 10-15 ul of the recommended dilution is used per data point. In WB this detects an ~33-35 kDa protein doublet, corresponding to the apparent molecular mass of the p33 and p35 forms of human CD74. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD74
Alternate Names
CD74, CLIP, DHLAG, HLADG, Ia-gamma, INVG34
Gene Symbol
CD74
Additional CD74 Products
Product Documents for CD74 Antibody (PIN.1) - Azide and BSA Free
Product Specific Notices for CD74 Antibody (PIN.1) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...