CD8 Antibody (OX-8) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12523
![Flow Cytometry: CD8 Antibody (OX-8) - BSA Free [NBP2-12523]](https://resources.bio-techne.com/images/products/CD8-Antibody-OX-8-Flow-Cytometry-NBP2-12523-img0002.jpg "Flow Cytometry: CD8 Antibody (OX-8) - BSA Free [NBP2-12523]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Rat
Applications
Validated:
Block/Neutralize, CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Flow Cytometry, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # OX-8
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
CD8 Antibody (OX-8) was developed against high Mw glycoproteins from rat thymocytes
Specificity
CD8 Antibody (OX-8) recognizes the hinge-like membrane-proximal domain of rat CD8a (32-34 kDa; alpha chain of the CD8 antigen).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CD8 Antibody (OX-8) - BSA Free
Flow Cytometry: CD8 Antibody (OX-8) - BSA Free [NBP2-12523]
Flow Cytometry: CD8 Antibody (OX-8) [NBP2-12523] - Separation of rat CD8a positive thymocytes (red-filled) from CD8a negative thymocytes (black-dashed) in flow cytometry analysis (surface staining) of rat thymocyte suspension stained using anti-rat CD8 (OX-8) purified antibody (concentration in sample 0.32 ug/ml) GAM APC.![Flow Cytometry: CD8 Antibody (OX-8) - BSA Free [NBP2-12523]](https://resources.bio-techne.com/images/products/CD8-Antibody-OX-8-Flow-Cytometry-NBP2-12523-img0001.jpg "Flow Cytometry: CD8 Antibody (OX-8) - BSA Free [NBP2-12523]")
Flow Cytometry: CD8 Antibody (OX-8) - BSA Free [NBP2-12523]
Flow Cytometry: CD8 Antibody (OX-8) [NBP2-12523] - Flow cytometry surface staining pattern of rat thymocyte suspension stained using anti-rat CD8 (OX-8) purified antibody (concentration in sample 0.32 ug/ml) GAM APC.Applications for CD8 Antibody (OX-8) - BSA Free
Application
Recommended Usage
Flow Cytometry
1-4 ug/ml
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CD8
Given its role in the immune system, CD8-deficiency in T-cells is a hallmark of many diseases and pathologies (8-10). Specifically, CD8+ T-cell deficiency is prevalent in chronic autoimmune diseases including multiple sclerosis, rheumatoid arthritis, ulcerative colitis, Crohn's disease, type 1 diabetes mellitus, and Graves' disease (8). Furthermore, cancers or chronic infection can lead to CD8 T-cell exhaustion as the continual antigen presentation and inflammatory signals eventually cause the CD8+ T-cells to lose functionality (9, 10). However, animal models and clinical studies have suggested that T-cells are capable of being reinvigorated using inhibitory receptor blockade resulting in better disease outcomes and these exhausted T-cells may be a potential therapeutic target (9, 10).
Alternative names for CD8 includes CD antigen: CD8a, CD8 antigen, alpha polypeptide (p32), CD8a molecule, CD8A, Leu2 T-lymphocyte antigen, LEU2, MAL, OKT8 T-cell antigen, p32, T cell co-receptor, T8 T-cell antigen, T-cell antigen Leu2, T-cell surface glycoprotein CD8 alpha chain, and T-lymphocyte differentiation antigen T8/Leu-2.
References
1. Littman D. R. (1987). The structure of the CD4 and CD8 genes. Annual review of immunology. https://doi.org/10.1146/annurev.iy.05.040187.003021
2. Naeim F. (2008). Chapter 2- Principles of Immunophenotyping. Hematopathology. https://doi.org/10.1016/B978-0-12-370607-2.00002-8.
3. Gao, G. F., & Jakobsen, B. K. (2000). Molecular interactions of coreceptor CD8 and MHC class I: the molecular basis for functional coordination with the T-cell receptor. Immunology today. https://doi.org/10.1016/s0167-5699(00)01750-3
4. UniProt (P01732)
5. UniProt (P01731)
6. Kappes D. J. (2007). CD4 and CD8: hogging all the Lck. Immunity. https://doi.org/10.1016/j.immuni.2007.11.002
7. Gangadharan, D., & Cheroutre, H. (2004). The CD8 isoform CD8alphaalpha is not a functional homologue of the TCR co-receptor CD8alphabeta. Current opinion in immunology. https://doi.org/10.1016/j.coi.2004.03.015
8. Pender M. P. (2012). CD8+ T-Cell Deficiency, Epstein-Barr Virus Infection, Vitamin D Deficiency, and Steps to Autoimmunity: A Unifying Hypothesis. Autoimmune diseases. https://doi.org/10.1155/2012/189096
9. Kurachi M. (2019). CD8+ T cell exhaustion. Seminars in immunopathology. https://doi.org/10.1007/s00281-019-00744-5
10. Hashimoto, M., Kamphorst, A. O., Im, S. J., Kissick, H. T., Pillai, R. N., Ramalingam, S. S., Araki, K., & Ahmed, R. (2018). CD8 T Cell Exhaustion in Chronic Infection and Cancer: Opportunities for Interventions. Annual review of medicine. https://doi.org/10.1146/annurev-med-012017-043208
Alternate Names
CD8, CD8A
Gene Symbol
CD8A
Additional CD8 Products
Product Documents for CD8 Antibody (OX-8) - BSA Free
Product Specific Notices for CD8 Antibody (OX-8) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...