CD99 Antibody (SPM586) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48032
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Kappa Clone # SPM586
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CD99 Antibody (SPM586) - IHC-Prediluted
Immunogen
Purified E-rosette forming cells from human peripheral blood lymphocytes
Localization
Cell surface
Specificity
Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. This antigen is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewings sarcoma cells and primitive peripheral neuroectodermal tumors. This monoclonal antibody shows a very similar reactivity to other CD99 monoclonal antibodys (e.g. O13, 12E7, or HBA-71) and is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Marker
Ewing's Sarcoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgM Kappa
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for CD99 Antibody (SPM586) - IHC-Prediluted
Immunohistochemistry-Paraffin: CD99 Antibody (SPM586) - IHC-Prediluted [NBP2-48032]
Immunohistochemistry-Paraffin: CD99 Antibody (SPM586) - IHC-Prediluted [NBP2-48032] - Human Ewing's sarcoma stained with CD99 Monoclonal Antibody (SPM586).Applications for CD99 Antibody (SPM586) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Hu-chromosome location: 1q32.2
Molecular weight of antigen: 90-110kDa
Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Molecular weight of antigen: 90-110kDa
Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein L purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD99
Alternate Names
CD99, MIC2, pilr-1, PILR-L
Gene Symbol
CD99
Additional CD99 Products
Product Documents for CD99 Antibody (SPM586) - IHC-Prediluted
Product Specific Notices for CD99 Antibody (SPM586) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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