CD99 Antibody (SPM586) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11457
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Rat
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Kappa Clone # SPM586
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Purified E-rosette forming cells from human peripheral blood lymphocytes
Localization
Cell surface
Specificity
Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. This antigen is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewings sarcoma cells and primitive peripheral neuroectodermal tumors. This monoclonal antibody shows a very similar reactivity to other CD99 monoclonal antibodys (e.g. O13, 12E7, or HBA-71) and is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Marker
Ewing's Sarcoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgM Kappa
Description
1.0 mg/ml of antibody purified by Protein L. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-44693).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Applications for CD99 Antibody (SPM586) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
5 - 10ul/million cells in 0.1ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0ug/ml
Immunohistochemistry-Paraffin
1:50 - 1:100
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
This antibody is CyTOF ready.
Optimal dilution for a specific application should be determined.
This antibody is CyTOF ready.
Formulation, Preparation, and Storage
Purification
Protein L purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CD99
Alternate Names
CD99, MIC2, pilr-1, PILR-L
Gene Symbol
CD99
Additional CD99 Products
Product Documents for CD99 Antibody (SPM586) - Azide and BSA Free
Product Specific Notices for CD99 Antibody (SPM586) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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