CDC2/CDK1 [p Tyr15] Antibody (6P7X9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33570

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 6P7X9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CDC2/CDK1 Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around Y15 of human CDC2/CDK1.1).

Sequence:
GTYGV

Modification

p Tyr 15

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CDC2/CDK1 [p Tyr15] Antibody (6P7X9)

CDC2/CDK1 [p Tyr15] Antibody (6P7X9)

Western Blot: CDC2/CDK1 [p Tyr15] Antibody (6P7X9) [NBP3-33570] -

Western Blot: CDC2/CDK1 [p Tyr15] Antibody (6P7X9) [NBP3-33570] - Western blot analysis of various lysates, using CDC2/CDK1 Rabbit mAb at1:2000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37C for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

Applications for CDC2/CDK1 [p Tyr15] Antibody (6P7X9)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CDC2/CDK1

The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.

Long Name

Cell Division Cycle 2/Cyclin-dependent Kinase 1

Alternate Names

CDK1

Gene Symbol

CDK1

Additional CDC2/CDK1 Products

Product Documents for CDC2/CDK1 [p Tyr15] Antibody (6P7X9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CDC2/CDK1 [p Tyr15] Antibody (6P7X9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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