CDC2/CDK1 Antibody (8F1)

Novus Biologicals, part of Bio-Techne | Catalog # H00000983-M04

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Lambda Clone # 8F1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CDC2/CDK1 Antibodies »

Product Specifications

Immunogen

CDC2 (AAH14563, 211 a.a. ~ 297 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

CDC2 - cell division cycle 2, G1 to S and G2 to M

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Lambda

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CDC2/CDK1 Antibody (8F1)

Western Blot: CDC2/CDK1 Antibody (8F1) [H00000983-M04]

Western Blot: CDC2/CDK1 Antibody (8F1) [H00000983-M04]

Western Blot: CDC2/CDK1 Antibody (8F1) [H00000983-M04] - Analysis of CDC2 expression in NIH/3T3 (Cat # L018V1).
Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (8F1) [H00000983-M04]

Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (8F1) [H00000983-M04]

Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (8F1) [H00000983-M04] - Analysis of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1.5 ug/ml
Western Blot: CDC2/CDK1 Antibody (8F1) [H00000983-M04]

Western Blot: CDC2/CDK1 Antibody (8F1) [H00000983-M04]

Western Blot: CDC2/CDK1 Antibody (8F1) [H00000983-M04] - Analysis of CDC2 expression in PC-12 (Cat # L012V1).
View 3 more images

Applications for CDC2/CDK1 Antibody (8F1)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CDC2/CDK1

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.

Long Name

Cell Division Cycle 2/Cyclin-dependent Kinase 1

Alternate Names

CDK1

Entrez Gene IDs

983 (Human)

Gene Symbol

CDK1

OMIM

116940 (Human)

UniProt

AAH14563 (Human)

Additional CDC2/CDK1 Products

Product Documents for CDC2/CDK1 Antibody (8F1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CDC2/CDK1 Antibody (8F1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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