CDC2/CDK1 Antibody (CDK1/873) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11507
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Bovine, Drosophila (Negative), Mink, Monkey, Mouse (Negative), Rat (Negative), S. pombe (Negative), Xenopus (Negative), Yeast (Negative)
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # CDK1/873
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for CDC2/CDK1 Antibody (CDK1/873) - Azide and BSA Free
Immunogen
Recombinant human full-length CDC2/CDK1 protein. (Uniprot: P06493)
Reactivity Notes
Does not react with Mouse, Rat, Xenopus, drosophila, S, pombe or S. cerevisiae.
Localization
Nuclear and Cytoplasmic
Specificity
Recognizes a 34kDa protein, which is identified as cyclin dependent kinase 1 (cdk1) or p34cdc2 protein kinase. cdk1 / p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated cdk1 / p34cdc2 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44915).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Applications for CDC2/CDK1 Antibody (CDK1/873) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
2 - 4 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CDC2/CDK1
Long Name
Cell Division Cycle 2/Cyclin-dependent Kinase 1
Alternate Names
CDK1
Gene Symbol
CDK1
Additional CDC2/CDK1 Products
Product Documents for CDC2/CDK1 Antibody (CDK1/873) - Azide and BSA Free
Product Specific Notices for CDC2/CDK1 Antibody (CDK1/873) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...